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Structure basis of non-structural protein pA151R from African Swine Fever Virus
- Source :
- Biochemical and Biophysical Research Communications. 532:108-113
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- African Swine Fever Virus (ASFV) is an enveloped double-stranded DNA icosahedral virus that causes the devastating hemorrhagic fever of pigs. ASFV infections severely impact swine production and cause an enormous economic loss, but no effective vaccine and therapeutic regimen is available. pA151R is a non-structural protein of ASFV, which is expressed at both early and late stages of viral infection. Significantly, pA151R may play a key role in ASFV replication and virus assembly as suppressing pA151R expression can reduce virus replication. However, little is known about the functional and structural mechanisms of pA151R because it shares a very low sequence identity to known structures. It was proposed that pA151R might participate in the redox pathway owing to the presence of a thioredoxin active site feature, the WCTKC motif. In this study, we determined the crystal structure of pA151R. Based on the crystal structure, we found that pA151R comprises of a central five-stranded β-sheet packing against two helices on one side and an incompact C-terminal region containing the WCTKC motif on the other side. Notably, two cysteines in the WCTKC motif, an additional cysteine C116 from the β7-β8 loop together with ND1 of H109 coordinate a Zn2+ ion to form a Zn-binding motif. These findings suggest that the structure of pA151R is significantly different from that of typical thioredoxins. Our structure should provide molecular insights into the understanding of functional and structural mechanisms of pA151R from ASFV and shall benefit the development of prophylactic and therapeutic anti-ASFV agents.
- Subjects :
- Models, Molecular
0301 basic medicine
Genes, Viral
Protein Conformation
Swine
Static Electricity
Sus scrofa
Biophysics
Viral Nonstructural Proteins
Crystallography, X-Ray
Biochemistry
African swine fever virus
Virus
03 medical and health sciences
chemistry.chemical_compound
Thioredoxins
0302 clinical medicine
Animals
African Swine Fever
Molecular Biology
Binding Sites
biology
Structural protein
Active site
Cell Biology
biology.organism_classification
African Swine Fever Virus
Virology
Recombinant Proteins
030104 developmental biology
Viral replication
chemistry
Structural Homology, Protein
030220 oncology & carcinogenesis
biology.protein
Protein Multimerization
Thioredoxin
DNA
Cysteine
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 532
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....37cc03ba5979bcfbce29ae905c9e2caf