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Cytotoxic Effects of Artemisia annua L. and Pure Artemisinin on the D-17 Canine Osteosarcoma Cell Line
- Source :
- Oxidative Medicine and Cellular Longevity, Vol 2019 (2019)
- Publication Year :
- 2019
-
Abstract
- Artemisia annua has been used for centuries in Traditional Chinese Medicine. Although used as an antimalarial drug, its active compound artemisinin and the semisynthetic derivatives have also been investigated for their anticancer properties, with interesting and promising results. The aims of this research were to evaluate (i) the cytotoxicity and the antiproliferative effect of pure artemisinin and a hydroalcoholic extract obtained from A. annua on the D-17 canine osteosarcoma cell line and (ii) the intracellular iron concentration and its correlation with the cytotoxic effects. Both artemisinin and hydroalcoholic extract induced a cytotoxic effect in a dose-dependent manner. Pure artemisinin caused an increase of cells in the S phase, whereas the hydroalcoholic extract induced an evident increase in the G2/M phase. A significant decrease of iron concentration was measured in D-17 cells treated with pure artemisinin and hydroalcoholic extract compared to untreated cells. In conclusion, although preliminary, the data obtained in this study are indicative of a more potent cytotoxic activity of the hydroalcoholic extract than pure artemisinin, indicating a possible synergistic effect of the phytocomplex and a mechanism of action involving iron and possibly ferroptosis. Considering the similarities between human and canine osteosarcomas, progress in deepening knowledge and improving therapeutic protocols will probably be relevant for both species, in a model of reciprocal translational medicine.
- Subjects :
- 0301 basic medicine
Aging
Article Subject
Artemisia annua
Pharmacology
Biochemistry
Canine Osteosarcoma
Artemisia annua, artemisinin, citotoxicity, iron, in vitro model, dog
03 medical and health sciences
0302 clinical medicine
parasitic diseases
medicine
Cytotoxic T cell
Artemisinin
lcsh:QH573-671
Cytotoxicity
biology
Chemistry
lcsh:Cytology
Cell Biology
General Medicine
biology.organism_classification
030104 developmental biology
Mechanism of action
Cell culture
030220 oncology & carcinogenesis
medicine.symptom
Intracellular
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity, Vol 2019 (2019)
- Accession number :
- edsair.doi.dedup.....3792bc4152d01082e4bf467cfcf8b85d