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Effects of isoflurane exposure during pregnancy on postnatal memory and learning in offspring rats
- Source :
- Molecular biology reports. 39(4)
- Publication Year :
- 2011
-
Abstract
- Emerging evidence has demonstrated that exposure to anesthetics early in life caused neurohistopathologic changes and persistent behavioral impairments. In this study, a maternal fetal rat model was developed to study the effects of isoflurane exposure during pregnancy on postnatal memory and learning in the offspring. Pregnant rats at gestational day 14 were either exposed to 1.3% isoflurane in a humidified 100% oxygen carrier gas or simply humidified 100% oxygen without any inhalational anesthetic for 2 h every day before delivery. Four weeks later, spatial learning and memory of the offspring were examined using the Morris Water Maze. The expression levels of GAP-43 and NPY in the hippocampal CA1 region of the pups were determined by immunohistochemistry and RT-PCR. Simultaneously, the ultrastructure changes in synapse of the hippocampus were also observed by transmission electron microscopy (TEM). Isoflurane exposure during pregnancy impaired postnatal spatial memory and learning in the offspring as shown by the longer escape latency and the fewer original platform crossings in the Morris Water Maze test. The number and optical densities of GAP-43 and NPY positive cells, as well as the levels of GAP-43 and NPY mRNA, decreased significantly in the hippocampus of isoflurane-exposed pups. Furthermore, TEM studies showed remarkable changes in synaptic ultrastructure of hippocampus. These results indicate that isoflurane exposure during pregnancy could cause postnatal spatial memory and learning impairments in offspring rats, which may be partially explained by the down-regulation of GAP-43 and NPY in the hippocampal area.
- Subjects :
- medicine.medical_specialty
Offspring
Morris water navigation task
Hippocampus
Hippocampal formation
Synapse
Rats, Sprague-Dawley
GAP-43 Protein
Memory
Pregnancy
Internal medicine
Genetics
medicine
Animals
Neuropeptide Y
RNA, Messenger
Maze Learning
Molecular Biology
Isoflurane
business.industry
Gene Expression Regulation, Developmental
General Medicine
medicine.disease
Immunohistochemistry
Rats
Endocrinology
Animals, Newborn
Prenatal Exposure Delayed Effects
Synapses
Gestation
Female
business
medicine.drug
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 39
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....377e8728d6e382109ab4fde7a9eb4b4a