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Retinoic Acid Induces Embryonic Stem Cells (ESCs) Transition to 2 Cell-Like State Through a Coordinated Expression of Dux and Duxbl1
- Source :
- Frontiers in Cell and Developmental Biology, Vol 7 (2020), Frontiers in Cell and Developmental Biology, Front Cell Dev Biol 7 (2020). doi:10.3389/fcell.2019.00385, info:cnr-pdr/source/autori:Tagliaferri D.; Mazzone P.; Noviello T.M.R.; Addeo M.; Angrisano T.; Del Vecchio L.; Visconte F.; Ruggieri V.; Russi S.; Caivano A.; Cantone I.; De Felice M.; Ceccarelli M.; Cerulo L.; Falco G./titolo:Retinoic Acid Induces Embryonic Stem Cells (ESCs) Transition to 2 Cell-Like State Through a Coordinated Expression of Dux and Duxbl1/doi:10.3389%2Ffcell.2019.00385/rivista:Front Cell Dev Biol/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume:7
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Embryonic stem cells (ESCs) are derived from inner cell mass (ICM) of the blastocyst. In serum/LIF culture condition, they show variable expression of pluripotency genes that mark cell fluctuation between pluripotency and differentiation metastate. The ESCs subpopulation marked by zygotic genome activation gene (ZGA) signature, including Zscan4, retains a wider differentiation potency than epiblast-derived ESCs. We have recently shown that retinoic acid (RA) significantly enhances Zscan4 cell population. However, it remains unexplored how RA initiates the ESCs to 2-cell like reprogramming. Here we found that RA is decisive for ESCs to 2C-like cell transition, and reconstructed the gene network surrounding Zscan4. We revealed that RA regulates 2C-like population co-activating Dux and Duxbl1. We provided novel evidence that RA dependent ESCs to 2C-like cell transition is regulated by Dux, and antagonized by Duxbl1. Our suggested mechanism could shed light on the role of RA on ESC reprogramming.
- Subjects :
- 0301 basic medicine
Cell
Population
Retinoic acid
ESC
Biology
03 medical and health sciences
chemistry.chemical_compound
Cell and Developmental Biology
0302 clinical medicine
metastate
medicine
retinoic acid
Inner cell mass
Blastocyst
education
lcsh:QH301-705.5
reproductive and urinary physiology
Original Research
education.field_of_study
urogenital system
2-cell like
ESCs
pluripotency
Cell Biology
Embryonic stem cell
Cell biology
030104 developmental biology
medicine.anatomical_structure
chemistry
lcsh:Biology (General)
030220 oncology & carcinogenesis
embryonic structures
Maternal to zygotic transition
biological phenomena, cell phenomena, and immunity
Reprogramming
Developmental Biology
Subjects
Details
- Language :
- English
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cell and Developmental Biology
- Accession number :
- edsair.doi.dedup.....3749cda686f1ad16223289945fe20928