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Significant increase in circulating tumour cells in pulmonary venous blood during surgical manipulation in patients with primary lung cancer
- Source :
- Interactive CardioVascular and Thoracic Surgery. 18:775-783
- Publication Year :
- 2014
- Publisher :
- Oxford University Press (OUP), 2014.
-
Abstract
- OBJECTIVES: Circulating tumour cells (CTCs) are tumour cells shed from a primary tumour and circulate in the peripheral blood after passing through the drainage vein. In previous studies, we showed that high numbers of CTCs were detected in the drainage pulmonary venous blood of most patients with resectable primary lung cancer, whereas only low numbers of CTCs were detected in the peripheral blood of some patients. Accordingly, this prospective study was conducted to assess changes in CTCs in the drainage pulmonary vein (PV) during lung cancer surgery. METHODS: A total of 30 consecutive peripheral-type primary lung cancer patients who underwent lobectomy (or right upper and middle bilobectomy) through open thoracotomy were included. For each patient, 2.5 ml of blood was sampled from the lobar PV of the primary tumour site before and after surgical manipulation for lobectomy. The CTCs were evaluated quantitatively with the CellSearch® system. RESULTS: Before surgical manipulation, CTCs were detected in PV blood in the majority of patients (22 of 30, 73.3%), although CTCs were detected in peripheral blood in only two patients (6.7%). The median number of CTCs in the PV (pvCTC-count) before surgical manipulation was 4.0 cells/2.5 ml, and there was no significant correlation between pvPV-count and any clinicopathological characteristic, including tumour size, progression and histological type. After surgical manipulation, at the time of completion of the lobectomy, the pvCTC-count significantly increased (median, 60.0 cells/2.5 ml; P= 0.001). The increase in pvCTC-count was significantly associated with microscopic lymphatic tumour invasion (ly); pvCTC-count significantly increased in ly-positive patients (pvCTC-count before and after surgical manipulation, 4.0 and 90.5 cells/2.5 ml, respectively; P= 0.006), but not in ly-negative patients (3.5 and 7.0 cells/2.5 ml, respectively; P= 0.153). The increase in pvCTC-count was not significantly associated with any other clinicopathological factor or with any surgical procedure, including the sequence of vessel interruption. CONCLUSIONS: We documented a significant increase in CTC count in drainage PV blood after surgical manipulation, especially in tumours with lymphatic invasion. We are awaiting survival data at 5 year follow-up examination, which may provide clinical significance of the pvCTC-count.
- Subjects :
- Adult
Male
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Pathology
Lung Neoplasms
Time Factors
Lymphovascular invasion
Urology
Lymphatic System
Circulating tumor cell
medicine
Humans
Neoplasm Invasiveness
Prospective Studies
Pneumonectomy
Lung cancer
Vein
Aged
Aged, 80 and over
Lung cancer surgery
Lung
business.industry
Venous blood
Middle Aged
Neoplastic Cells, Circulating
medicine.disease
Treatment Outcome
medicine.anatomical_structure
Lymphatic system
Pulmonary Veins
Lymphatic Metastasis
Female
Surgery
Cardiology and Cardiovascular Medicine
business
Subjects
Details
- ISSN :
- 15699285 and 15699293
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Interactive CardioVascular and Thoracic Surgery
- Accession number :
- edsair.doi.dedup.....3741bcf86eef54b8a5269bca294ed646