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Targeted disruption of hir1 alters the transcriptional expression pattern of putative lignocellulolytic genes in the white-rot fungus Pleurotus ostreatus
- Source :
- Fungal genetics and biology : FGB. 147
- Publication Year :
- 2020
-
Abstract
- Pleurotus ostreatus is frequently used in molecular genetics and genomic studies on white-rot fungi because various molecular genetic tools and relatively well-annotated genome databases are available. To explore the molecular mechanisms underlying wood lignin degradation by P. ostreatus, we performed mutational analysis of a newly isolated mutant UVRM28 that exhibits decreased lignin-degrading ability on the beech wood sawdust medium. We identified that a mutation in the hir1 gene encoding a putative histone chaperone, which probably plays an important role in DNA replication-independent nucleosome assembly, is responsible for the mutant phenotype. The expression pattern of ligninolytic genes was altered in hir1 disruptants. The most highly expressed gene vp2 was significantly inactivated, whereas the expression of vp1 was remarkably upregulated (300-400 fold) at the transcription level. Conversely, many cellulolytic and xylanolytic genes were upregulated in hir1 disruptants. Chromatin immunoprecipitation analysis suggested that the histone modification status was altered in the 5'-upstream regions of some of the up- and down-regulated lignocellulolytic genes in hir1 disruptants compared with that in the 20b strain. Hence, our data provide new insights into the regulatory mechanisms of lignocellulolytic genes in P. ostreatus.
- Subjects :
- medicine.medical_specialty
Nucleosome assembly
Mutant
Gene Expression
Biology
Pleurotus
Microbiology
Lignin
Fungal Proteins
03 medical and health sciences
Transcription (biology)
Molecular genetics
Gene Expression Regulation, Fungal
Gene expression
Genetics
medicine
Wood degradation
Gene Silencing
Gene
030304 developmental biology
0303 health sciences
030306 microbiology
Wood
Cell biology
Histone
biology.protein
White rot
Basidiomycete
Histone modification
Chromatin immunoprecipitation
Subjects
Details
- ISSN :
- 10960937
- Volume :
- 147
- Database :
- OpenAIRE
- Journal :
- Fungal genetics and biology : FGB
- Accession number :
- edsair.doi.dedup.....372d241b1835a017ee9ec248acc21e15