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Chemometric approach to the treatment of benzodiazepine separation and peak broadening in capillary electrophoresis
- Source :
- Journal of chromatography. A. 849(2)
- Publication Year :
- 1999
-
Abstract
- A chemometric methodology was used to study capillary efficiency and the separation of ten benzodiazepines in capillary electrophoresis. The resolution between two adjacent peaks on the electropherogram was estimated and the overall quality of the separation was assessed by means of a new response function. The nature (methanol or acetonitrile) and proportion of the organic modifier both in the background electrolyte and the sample buffer and the injection time were considered. The results predicted that if the sample had a lower dielectric constant than the background electrolyte buffer then a much larger injection volume could be used. The computer optimization routine was experimentally validated and the result demonstrated that the fastest electrophoretic reparation was obtained with acetonitrile (7 min instead of 9 min with methanol).
- Subjects :
- Chromatography
Acetonitriles
Time Factors
Resolution (mass spectrometry)
Capillary action
Methanol
Organic Chemistry
Analytical chemistry
Electrophoresis, Capillary
General Medicine
Electrolyte
Biochemistry
Analytical Chemistry
Chemometrics
Electropherogram
chemistry.chemical_compound
Electrophoresis
Benzodiazepines
Electrolytes
Capillary electrophoresis
chemistry
Acetonitrile
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 849
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography. A
- Accession number :
- edsair.doi.dedup.....3700467c61573328a71688dbc31a71e5