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The nuclear localization of WAP and CSN genes is modified by lactogenic hormones in HC11 cells
- Source :
- Journal of Cellular Biochemistry, Journal of Cellular Biochemistry, Wiley, 2008, 105 (1), pp.262-270. ⟨10.1002/jcb.21823⟩
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- International audience; Whey acidic protein (WAP) and casein (CSN) genes are among the most highly expressed milk protein genes in the mammary gland of the lactating mouse. Their tissue-specific regulation depends on the activation and recruitment of transcription factors, and chromatin modifications in response to hormonal stimulation. We have investigated if another mechanism, such as specific positioning of the genes in the nucleus, could be involved in their functional regulation. Fluorescent in situ hybridization was used to study the nuclear localization of WAP and CSN genes in mouse mammary epithelial cells (HC11) cultured in the absence and presence of lactogenic hormones. Automatic 3D image processing and analysis tools were developed to score gene positions. In the absence of lactogenic hormones, both genes are distributed non-uniformly within the nucleus: the CSN locus was located close to the nuclear periphery and the WAP gene tended to be central. Stimulation by lactogenic hormones induced a statistically significant change to their distance from the periphery, which has been described as a repressive compartment. The detection of genes in combination with the corresponding chromosome-specific probe revealed that the CSN locus is relocated outside its chromosome territory following hormonal stimulation, whereas the WAP gene, which is already sited more frequently outside its chromosome territory in the absence of hormones, is not affected. We conclude that milk protein genes are subject to nuclear repositioning when activated, in agreement with a role for nuclear architecture in gene regulation, but that they behave differently as a function of their chromosomal context.
- Subjects :
- Active Transport, Cell Nucleus
PROLACTIN
Locus (genetics)
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biochemistry
Chromosomes
Cell Line
Mice
03 medical and health sciences
0302 clinical medicine
CHROMOSOME TERRITORIES
Heterochromatin
medicine
Animals
Lactation
Molecular Biology
Transcription factor
Gene
030304 developmental biology
Cell Nucleus
Regulation of gene expression
0303 health sciences
3D FISH
biology
Caseins
Cell Biology
Milk Proteins
Molecular biology
Hormones
Chromatin
WHEY ACIDIC PROTEIN
Cell nucleus
medicine.anatomical_structure
030220 oncology & carcinogenesis
biology.protein
CASEIN
Whey Acidic Protein
Nuclear localization sequence
Subjects
Details
- ISSN :
- 07302312 and 10974644
- Volume :
- 105
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....36fe7c370b618f6a387872e8c7bd4a54