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Membrane Interactions of Natural Cyclic Lipodepsipeptides of the Viscosin Group
- Source :
- Biochimica et Biophysica Acta:Biomembranes, Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2017, 1859 (3), pp.331-339. ⟨10.1016/j.bbamem.2016.12.013⟩, Biochimica et Biophysica Acta (BBA)-Biomembranes, 1859(3), 331-339. Elsevier B.V.
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- Many Pseudomonas spp. produce cyclic lipodepsipeptides (CLPs), which, besides their role in biological functions such as motility, biofilm formation and interspecies interactions, are antimicrobial. It has been established that interaction with the cellular membrane is central to the mode of action of CLPs. In this work, we focus on the CLPs of the so-called viscosin group, aiming to assess the impact of the main structural variations observed within this group on both the antimicrobial activity and the interaction with model membranes. The antimicrobial activity of viscosin, viscosinamide A, WLIP and pseudodesmin A were all tested on a broad panel of mainly Gram-positive bacteria. Their capacity to permeabilize or fuse PG/PE/cardiolipin model membrane vesicles is assessed using fluorescent probes. We find that the Glu2/Gln2 structural variation within the viscosin group is the main factor that influences both the membrane permeabilization properties and the minimum inhibitory concentration of bacterial growth, while the configuration of the Leu5 residue has no apparent effect. The CLP-membrane interactions were further evaluated using CD and FT-IR spectroscopy on model membranes consisting of PG/PE/cardiolipin or POPC with or without cholesterol. In contrast to previous studies, we observe no conformational change upon membrane insertion. The CLPs interact both with the polar heads and aliphatic tails of model membrane systems, altering bilayer fluidity, while cholesterol reduces CLP insertion depth.
- Subjects :
- 0301 basic medicine
spectroscopy
Conformational change
Magnetic Resonance Spectroscopy
model membranes
030106 microbiology
Lipid Bilayers
Biophysics
cyclic lipodepsipeptide
Microbial Sensitivity Tests
Biology
Gram-Positive Bacteria
Biochemistry
Peptides, Cyclic
Chemical communication
Permeability
03 medical and health sciences
chemistry.chemical_compound
Lipopeptides
Biosynthesis
Bacterial Proteins
Pseudomonas
Gram-Negative Bacteria
Spectroscopy, Fourier Transform Infrared
viscosin group
Cardiolipin
POPC
ComputingMilieux_MISCELLANEOUS
antimicrobial activity
Bilayer
Circular Dichroism
Biofilm
Biological membrane
Cell Biology
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics
030104 developmental biology
Membrane
chemistry
international
membrane permeabilization
Subjects
Details
- Language :
- English
- ISSN :
- 00052736 and 18792642
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta:Biomembranes, Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2017, 1859 (3), pp.331-339. ⟨10.1016/j.bbamem.2016.12.013⟩, Biochimica et Biophysica Acta (BBA)-Biomembranes, 1859(3), 331-339. Elsevier B.V.
- Accession number :
- edsair.doi.dedup.....36ea6748682cc04e1c9f9c6bad9e618b