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Microsphere-Based Nanoindentation for the Monitoring of Cellular Cortical Stiffness Regulated by MT1-MMP
- Source :
- Small. 14:1803000
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Biophysical properties are intimately connected to metastatic functions and aggressiveness in cancers. Especially, cellular stiffness is regarded as a biomarker for the understanding of metastatic potential and drug sensitivity. Here, protease-mediated changes of cortical stiffness are identified due to the deformation of cytoskeleton alignment at a cortex. For the past few decades, membrane type 1-matrix metalloproteinase (MT1-MMP) has been well known as a kernel protease enriched in podosomes during metastasis for extracellular matrix degradation. However, the biophysical significance of MT1-MMP expressing cancer cells is still unknown. Therefore, the nanomechanics of cancer cells is analyzed by a nanoindentation using a microsphere-attached cantilever of atomic force microscopy (AFM). In conclusion, the results suggest that MT1-MMP has contributed as a key regulator in cytoskeletal deformation related with cancer metastasis. Particularly, the AFM-based nanoindentation system for the monitoring of cortical nanomechanics will be crucial to understand molecular networks in cancers.
- Subjects :
- 0301 basic medicine
Podosome
Chemistry
technology, industry, and agriculture
macromolecular substances
General Chemistry
Matrix metalloproteinase
Nanoindentation
Microscopy, Atomic Force
medicine.disease
Microspheres
Metastasis
Cell biology
Biomaterials
03 medical and health sciences
030104 developmental biology
Cancer cell
Matrix Metalloproteinase 14
medicine
General Materials Science
Cytoskeleton
Extracellular Matrix Degradation
Nanomechanics
Biotechnology
Subjects
Details
- ISSN :
- 16136810
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Small
- Accession number :
- edsair.doi.dedup.....36e90b734cacadd052874c16a57cce8c