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HRF, a putative basic helix-loop-helix-PAS-domain transcription factor is closely related to hypoxia-inducible factor-1 alpha and developmentally expressed in blood vessels
- Source :
- Mechanisms of development. 63(1)
- Publication Year :
- 1997
-
Abstract
- Transcription factors of the bHLH-PAS protein family are important regulators of developmental processes such as neurogenesis and tracheal development in invertebrates. Recently a bHLH-PAS protein, named trachealess (trl) was identified as a master regulator of tracheogenesis. Hypoxia-inducible factor, HIF-1 alpha, is a vertebrate relative of trl which is likely to be involved in growth of blood vessels by the induction of vascular endothelial growth factor (VEGF) in response to hypoxia. In the present study we describe mRNA cloning and mRNA expression pattern of mouse HIF-related factor (HRF), a novel close relative of HIF-1 alpha which is expressed most prominently in brain capillary endothelial cells and other blood vessels as well as in bronchial epithelium in the embryo and the adult. In addition, smooth muscle cells of the uterus, neurons, brown adipose tissue and various epithelial tissues express HRF mRNA as well. High expression levels of HRF mRNA in embryonic choroid plexus and kidney glomeruli, places where VEGF is highly expressed, suggest a role of this factor in VEGF gene activation similar to that of HIF-1 alpha. Given the similarity between morphogenesis of the tracheal system and the vertebrate vascular system, the expression pattern of HRF in the vasculature and the bronchial tree raises the possibility that this family of transcription factors may be involved in tubulogenesis.
- Subjects :
- Transcriptional Activation
medicine.medical_specialty
Embryology
Transcription, Genetic
Molecular Sequence Data
Morphogenesis
Biology
Polymerase Chain Reaction
chemistry.chemical_compound
Mice
Internal medicine
medicine
Basic Helix-Loop-Helix Transcription Factors
Animals
Humans
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Transcription factor
DNA Primers
Regulation of gene expression
Messenger RNA
Basic helix-loop-helix
Base Sequence
Sequence Homology, Amino Acid
Neurogenesis
Helix-Loop-Helix Motifs
Brain
Gene Expression Regulation, Developmental
Nuclear Proteins
Hypoxia-Inducible Factor 1, alpha Subunit
Cell biology
Capillaries
Vascular endothelial growth factor
DNA-Binding Proteins
Endocrinology
Hypoxia-inducible factors
chemistry
Organ Specificity
Protein Biosynthesis
Vertebrates
Female
Endothelium, Vascular
Hypoxia-Inducible Factor 1
Sequence Alignment
Developmental Biology
Transcription Factors
Subjects
Details
- ISSN :
- 09254773
- Volume :
- 63
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Mechanisms of development
- Accession number :
- edsair.doi.dedup.....36e367f83b106d5906a4e7174e8e524a