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Prognostic effect of epidermal growth factor receptor gene mutations and the aberrant phosphorylation of Akt and ERK in ovarian cancer
- Publication Year :
- 2011
- Publisher :
- Landes Bioscience, 2011.
-
Abstract
- We herein assessed the influence of Epidermal Growth Factor Receptor (EGFR) gene mutations on EGFR expression levels, downstream mediators such as Akt or ERK, and overall survival in patients with ovarian cancer.EGFR mutation status was analyzed by direct sequencing in 102 Japanese ovarian cancer patients. The EGFR expression, phosphorylated Akt (pAkt), and phosphorylated ERK (pERK) were determined by immunohistochemistry.Twenty-nine EGFR gene mutations were detected in 24 of 102 patinets (23.5%). EGFR mutations were observed in 27.9% (19/68) in serous adenocarcinomas, 15.0% (3/20) in clear cell adenocarcinomas, and 66.7% (2/3) in mucinous adenocarcinomas, while no mutations were observed in endometrioid adenocarcinomas (0/11). Protein expression of EGFR, pAkt, and pERK were detected in 47 (46.1%), 49 (48%), and 17 (16.7%) of patients, respectively. EGFR gene mutations, EGFR and pERK expression were not associated with a poor prognosis. In a multivariate analysis, a High pAkt expression was found to be a significant predictor for both the progression free survival (p=0.017) and overall survival (P=0.025).EGFR gene mutations were frequently observed in not only non-small-cell lung cancer (NSCLC), but also in ovarian cancer in Japanese patients. the selective EGFR inhibitor Gefitinib might therefore offer some benefit in patients with EGFR mutations in ovarian cancer. Our results indicate that the Akt, but not necessarily EGFR, is one of the most important target in the response of the platinum-based chemotherapy and prognosis for ovarian cancer patients.
- Subjects :
- MAPK/ERK pathway
Cancer Research
Gene mutation
medicine.disease_cause
Disease-Free Survival
Growth factor receptor
medicine
Humans
ERBB3
Epidermal growth factor receptor
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Protein kinase B
Pharmacology
Ovarian Neoplasms
Mutation
biology
business.industry
medicine.disease
Molecular biology
Immunohistochemistry
ErbB Receptors
Oncology
Multivariate Analysis
Cancer research
biology.protein
Molecular Medicine
Female
Ovarian cancer
business
Proto-Oncogene Proteins c-akt
Research Paper
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....36c950fea49248b5f014295fb68198bc