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Interleukin-1β induced vascular permeability is dependent on induction of endothelial Tissue Factor (TF) activity
- Source :
- Journal of Translational Medicine, Vol 3, Iss 1, p 37 (2005), Journal of Translational Medicine
- Publication Year :
- 2005
- Publisher :
- BMC, 2005.
-
Abstract
- IL-1β is a pleotropic cytokine that may mediate increased procoagulant activity and permeability in endothelial tissue during inflammatory conditions. The procoagulant effects of IL-1β are mediated through induction of tissue factor (TF) but its alterations on vascular permeability are not well characterized. We found that IL-1β induced a rapid and dose-dependent increase in TF activity in human umbilical vein endothelial cells (ECs) under routine culture conditions. However, IL-1β caused a rapid and marked increase in permeability across confluent EC monolayers using a two-compartmentin vitromodel only in the presence of factor VIII-deficient plasma that was completely abrogated by neutralizing anti-TF antibody pre-treatment.In vitropermeability was associated with loss of EC surface expression of VE-cadherin and contraction of F-actin cytoskeletal elements that resulted in EC intercellular gap formation. These data demonstrate that IL-1β induces marked changes in permeability across activated endothelium via a TF dependent mechanism and suggest that modulation of TF activity may represent a strategy to treat various acute and chronic inflammatory conditions mediated by this cytokine.
- Subjects :
- videomicroscopy
Endothelium
business.industry
medicine.medical_treatment
Research
lcsh:R
lcsh:Medicine
Inflammation
Vascular permeability
General Medicine
neovasculature
General Biochemistry, Genetics and Molecular Biology
In vitro
Umbilical vein
Cell biology
Tissue factor
Cytokine
medicine.anatomical_structure
Permeability (electromagnetism)
inflammation
Immunology
Medicine
endothelial tissue
medicine.symptom
business
Subjects
Details
- Language :
- English
- ISSN :
- 14795876
- Volume :
- 3
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Translational Medicine
- Accession number :
- edsair.doi.dedup.....36c1ff03f78ce499e910b3899731d454