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Identification of an epigenetic biomarker panel with high sensitivity and specificity for colorectal cancer and adenomas
- Source :
- Molecular Cancer, Vol 10, Iss 1, p 85 (2011), Molecular Cancer
- Publication Year :
- 2011
-
Abstract
- Background The presence of cancer-specific DNA methylation patterns in epithelial colorectal cells in human feces provides the prospect of a simple, non-invasive screening test for colorectal cancer and its precursor, the adenoma. This study investigates a panel of epigenetic markers for the detection of colorectal cancer and adenomas. Methods Candidate biomarkers were subjected to quantitative methylation analysis in test sets of tissue samples from colorectal cancers, adenomas, and normal colonic mucosa. All findings were verified in independent clinical validation series. A total of 523 human samples were included in the study. Receiver operating characteristic (ROC) curve analysis was used to evaluate the performance of the biomarker panel. Results Promoter hypermethylation of the genes CNRIP1, FBN1, INA, MAL, SNCA, and SPG20 was frequent in both colorectal cancers (65-94%) and adenomas (35-91%), whereas normal mucosa samples were rarely (0-5%) methylated. The combined sensitivity of at least two positives among the six markers was 94% for colorectal cancers and 93% for adenoma samples, with a specificity of 98%. The resulting areas under the ROC curve were 0.984 for cancers and 0.968 for adenomas versus normal mucosa. Conclusions The novel epigenetic marker panel shows very high sensitivity and specificity for both colorectal cancers and adenomas. Our findings suggest this biomarker panel to be highly suitable for early tumor detection.
- Subjects :
- Adenoma
Adult
Male
Oncology
Cancer Research
medicine.medical_specialty
Colorectal cancer
Biology
Sensitivity and Specificity
lcsh:RC254-282
Epigenesis, Genetic
CNRIP1
Intestinal mucosa
Internal medicine
Biomarkers, Tumor
medicine
Carcinoma
Humans
FBN1
Epigenetics
Intestinal Mucosa
early detection
Aged
Aged, 80 and over
Receiver operating characteristic
Research
Gene Expression Profiling
Biomarker
Middle Aged
MAL
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Gene Expression Regulation, Neoplastic
DNA methylation
INA
Cancer research
Molecular Medicine
Biomarker (medicine)
Female
methylation
SNCA
SPG20
Colorectal Neoplasms
colorectal neoplasia
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Molecular Cancer, Vol 10, Iss 1, p 85 (2011), Molecular Cancer
- Accession number :
- edsair.doi.dedup.....36ae71bad2bddf0dd48ea95af42b7aa0