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Preparation of three-layered porous PLA/PEG scaffold: relationship between morphology, mechanical behavior and cell permeability
- Source :
- Journal of the mechanical behavior of biomedical materials. 54
- Publication Year :
- 2015
-
Abstract
- Interface tissue engineering (ITE) is used to repair or regenerate interface living tissue such as for instance bone and cartilage. This kind of tissues present natural different properties from a biological and mechanical point of view. With the aim to imitating the natural gradient occurring in the bone-cartilage tissue, several technologies and methods have been proposed over recent years in order to develop polymeric functionally graded scaffolds (FGS). In this study three-layered scaffolds with a pore size gradient were developed by melt mixing polylactic acid (PLA) and two water-soluble porogen agents: sodium chloride (NaCl) and polyethylene glycol (PEG). Pore dimensions were controlled by NaCl granulometry while PEG solvation created a micropores network within the devices. Scaffolds were characterized from a morphological and mechanical point of view in order to find a correlation between the preparation method, the pore architecture and compressive mechanical behavior. Biological tests were also performed in order to study the effect of pore size gradient on the permeation of different cell lines in co-culture. To imitate the physiological work condition, compressive tests were also performed in phosphate buffered saline (PBS) solution at 37 °C. The presented preparation method permitted to prepare three-layered scaffolds with high control of porosity and pore size distribution. Furthermore mechanical behaviors were found to be strongly affected by pore architecture of tested devices as well as the permeation of osteoblast and fibroblast in-vitro.
- Subjects :
- Materials science
Bone Regeneration
Cell Survival
Polymers
Particulate leaching
Polyesters
Biomedical Engineering
Biocompatible Materials
02 engineering and technology
Polyethylene glycol
010402 general chemistry
01 natural sciences
Permeability
Cell Line
Polyethylene Glycols
Biomaterials
chemistry.chemical_compound
Mice
Polylactic acid
Tissue engineering
Melt mixing
PEG ratio
Animals
Lactic Acid
Composite material
Bone regeneration
Porosity
Cell Proliferation
Mechanical Phenomena
chemistry.chemical_classification
Tissue Scaffolds
Interface tissue engineering
Pore size gradient
Adhesiveness
Water
Functionally graded scaffold
Polymer
Permeation
021001 nanoscience & nanotechnology
Biomaterial
0104 chemical sciences
Settore ING-IND/22 - Scienza E Tecnologia Dei Materiali
chemistry
Solubility
Mechanics of Materials
0210 nano-technology
Subjects
Details
- ISSN :
- 18780180
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Journal of the mechanical behavior of biomedical materials
- Accession number :
- edsair.doi.dedup.....36943c7335c7f50af00fdbecf5908b69