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Immunostimulatory Effects of Recombinant Erysipelothrix rhusiopathiae Expressing Porcine Interleukin-18 in Mice and Pigs
- Source :
- Clinical and Vaccine Immunology. 19:1393-1398
- Publication Year :
- 2012
- Publisher :
- American Society for Microbiology, 2012.
-
Abstract
- Interleukin-18 (IL-18), which was originally called gamma interferon (IFN-γ)-inducing factor, has been shown to play an important role in innate and acquired immune responses. In this study, attenuated Erysipelothrix rhusiopathiae strains were engineered to produce porcine IL-18 (poIL-18) and evaluated for their potential immunostimulatory effect in animals. Recombinant poIL-18 was successfully expressed in the recombinant E. rhusiopathiae strains YS-1/IL-18 and KO/IL-18. The culture supernatant of YS-1/IL-18 was confirmed to induce IFN-γ production in murine splenocytes in vitro , and this production was inhibited by incubation with anti-poIL-18 monoclonal antibodies. Furthermore, more IFN-γ production was induced upon stimulation of splenocytes with concanavalin A for splenocytes from mice that were intraperitoneally inoculated with YS-1/IL-18 than for splenocytes from control mice inoculated with the parent strain YS-1. Peritoneal macrophages from mice preinoculated with YS-1/IL-18 exhibited enhanced phagocytosis of Salmonella enterica subsp. enterica serovar Typhimurium compared with peritoneal macrophages from control mice preinoculated with YS-1. We also confirmed the immunostimulatory effect on humoral immune responses against antigens of E. rhusiopathiae and Mycoplasma hyopneumoniae in gnotobiotic pigs that were orally preinoculated with KO/IL-18. Thus, these results provide evidence that E. rhusiopathiae is a promising vector for the expression of host cytokines and suggest the potential utility of E. rhusiopathiae vector-encoded cytokines in the activation of host innate and acquired immune responses.
- Subjects :
- Microbiology (medical)
Swine
medicine.drug_class
Phagocytosis
Clinical Biochemistry
Immunology
Erysipelothrix rhusiopathiae
Vaccines, Attenuated
Monoclonal antibody
Microbiology
law.invention
Mice
Immune system
Antigen
Mycoplasma hyopneumoniae
law
medicine
Animals
Immunology and Allergy
Vaccines
Mice, Inbred BALB C
Vaccines, Synthetic
biology
Interleukin-18
biology.organism_classification
Salmonella enterica
Bacterial Vaccines
Erysipelothrix
Leukocytes, Mononuclear
Macrophages, Peritoneal
Recombinant DNA
Female
Spleen
Subjects
Details
- ISSN :
- 1556679X and 15566811
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Clinical and Vaccine Immunology
- Accession number :
- edsair.doi.dedup.....368daec017b8d4911cf2b3730112f2b2