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Screening of anti-lung cancer bioactive compounds from Curcuma longa by target cell extraction and UHPLC/LTQ Orbitrap MS
- Source :
- China Journal of Chinese Materia Medica.
- Publication Year :
- 2016
- Publisher :
- China Journal of Chinese Materia Medica, 2016.
-
Abstract
- A target cell extraction-chemical profiling method based on human alveolar adenocarcinoma cell line (A549 cells) and UHPLC/LTQ Orbitrap MS for screening the anti-lung cancer bioactive compounds from Curcuma longa has been developed in this paper. According to the hypothesis that when cells are incubated together with the extract of Curcuma longa, the potential bioactive compounds in the extract should selectively combine with the cells, then the cell-binding compounds could be separated and analyzed by LC-MS. The bioactive compounds in C. longa are lipophilic components. They intend to be absorbed on the inner wall of cell culture flask when they were incubated with A549 cells, which will produce interference in the blank solution. In this paper, by using cells digestion and multi-step centrifugation and transfer strategy, the interference problem has been solved. Finally, using the developed method, three cell-binding compounds were screened out and were identified as bisdemethoxycurcumin, demethoxycurcumin, and curcumin. These compounds are the main bioactive compounds with anti-lung cancer bioactivity in C. longa. The improved method developed in this paper could avoid the false positive results due to the absorption of lipophilic compounds on the inner wall of cell culture flask, which will to be an effective complementary method for current target cell extraction-chemical profiling technology.
- Subjects :
- 0301 basic medicine
Curcumin
Cell
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Curcuma
Liquid chromatography–mass spectrometry
Bisdemethoxycurcumin
medicine
Humans
Pharmacology (medical)
Centrifugation
General Pharmacology, Toxicology and Pharmaceutics
Chromatography, High Pressure Liquid
A549 cell
Chromatography
biology
Plant Extracts
010401 analytical chemistry
biology.organism_classification
Antineoplastic Agents, Phytogenic
0104 chemical sciences
030104 developmental biology
medicine.anatomical_structure
Complementary and alternative medicine
chemistry
A549 Cells
Cell culture
Chromatography, Liquid
Subjects
Details
- ISSN :
- 10015302
- Database :
- OpenAIRE
- Journal :
- China Journal of Chinese Materia Medica
- Accession number :
- edsair.doi.dedup.....367d5720aefbf8ce273027ceb05e9968