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Bile deficiency induces changes in intestinal glucose absorption in mice
- Source :
- Surgery. 160(6)
- Publication Year :
- 2016
-
Abstract
- Background Biliary tract obstruction is a common clinical problem. In this study, we attempted to understand the change in intestinal glucose absorption after biliary tract obstruction. Methods Experimental models of murine biliary duct ligation and external biliary drainage were established. Murine intestinal mucosal glucose absorption was examined with Ussing chambers according to the increase in the short-circuit current in vitro and blood glucose measurement after oral glucose in vivo. The protein expression of the sodium-glucose cotransporter (SGLT1) and the facilitated glucose transporter, member 2 (GLUT2) was analyzed by Western blot and immunohistochemistry. Results The results from Ussing chamber experiments showed that duodenal mucosal glucose absorption levels were significantly higher in biliary duct ligation and biliary drainage mice than those in normal control mice at 1 and 2 weeks after the operation. Gastrointestinal bile acid administration almost reversed the elevated duodenal mucosal glucose absorption to the normal level in biliary drainage mice. The results from the experiments in vivo further confirmed that the glucose absorption increased in biliary duct ligation and biliary drainage mice. The protein expression levels of SGLT1 in the duodenal mucosae of both biliary duct ligation and biliary drainage mice were markedly higher than those in control mice, and the protein expression of GLUT2 was not significantly altered, compared with control mice. Conclusion Bile deficiency in the intestine upregulates the expression of intestinal mucosal SGLT1 and enhances intestinal mucosal glucose absorption capacity, which contributes to the understanding of intestinal physiologic function for patients with biliary duct obstruction and external biliary drainage.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
medicine.drug_class
Duodenum
Carbohydrate metabolism
Gastroenterology
Bile Acids and Salts
03 medical and health sciences
Mice
Sodium-Glucose Transporter 1
Western blot
Gastrointestinal Agents
Internal medicine
Medicine
Animals
Intestinal Mucosa
Blood Glucose Measurement
Ligation
Glucose Transporter Type 2
Cholestasis
medicine.diagnostic_test
biology
Ussing chamber
Bile acid
business.industry
Glucose transporter
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
Endocrinology
Glucose
Intestinal Absorption
biology.protein
GLUT2
Surgery
business
Subjects
Details
- ISSN :
- 15327361
- Volume :
- 160
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Surgery
- Accession number :
- edsair.doi.dedup.....36782a9b80a1aefd72296e346f98910d