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Disc large 1 expression is altered by human papillomavirus E6/E7 proteins in organotypic cultures of human keratinocytes
- Source :
- Journal of General Virology, 97, 453-462. Society for General Microbiology, CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET, Bugnon Valdano, M, Cavatorta, A L, Morale, M G, Marziali, F, de Souza Lino, V, Steenbergen, R D M, Boccardo, E & Gardiol, D 2016, ' Disc large 1 expression is altered by human papillomavirus E6/E7 proteins in organotypic cultures of human keratinocytes ', Journal of General Virology, vol. 97, pp. 453-462 . https://doi.org/10.1099/jgv.0.000364
- Publication Year :
- 2015
-
Abstract
- Loss of cell polarity is a fundamental process in cell transformation. Among polarity proteins, we focused on human disc large (DLG1), which is localized mainly at adherens junctions and contributes to the control of cell proliferation. We previously demonstrated that its expression is altered in HPV-associated cervical neoplastic lesions, but the mechanisms beyond this remain unknown. In this study, we analysed the contribution of HPV proteins to the changes in DLG1 expression in the squamous epithelium. We observed tissue and intracellular misdistribution of DLG1 when high-risk HPV-18 E7 or E6/E7 proteins were expressed in organotypic raft cultures. The viral oncoproteins induce the loss of DLG1 from the cell borders and an increase in the level of DLG1 protein, reflecting the pattern observed in cervical lesions. These findings were corroborated in cultures bearing the entire HPV-18 genome. Interestingly, changes in tissue distribution and abundance of DLG1 were also detected in organotypic cultures expressing the low-risk HPV-11 E7 or E6/E7 proteins, suggesting a conserved function among different HPV types. However, for low-risk HPVs, the subcellular localization of DLG1 at cell-to-cell contacts was predominantly maintained. This report offers new evidence, we believe, of the involvement of HPV proteins in DLG1 expression pattern and our data support previous observations regarding DLG1 expression in cervical lesions. Fil: Bugnon Valdano, Marina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Cavatorta, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Morale, M. G.. Universidade de Sao Paulo; Brasil Fil: Marziali, Federico Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Lino, V. de Souza. Universidade de Sao Paulo; Brasil Fil: Steenbergen, R. D. M.. VU University Medical Center; Países Bajos Fil: Boccardo, E.. Universidade de Sao Paulo; Brasil Fil: Gardiol, Daniela Nora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
- Subjects :
- 0301 basic medicine
Keratinocytes
HPV
Otras Ciencias Biológicas
organotypic cultures
Cell
purl.org/becyt/ford/1 [https]
Ciencias Biológicas
Adherens junction
Discs Large Homolog 1 Protein
03 medical and health sciences
Virology
Cell polarity
medicine
Humans
polarity
purl.org/becyt/ford/1.6 [https]
Cells, Cultured
Adaptor Proteins, Signal Transducing
biology
Human papillomavirus 18
Cell growth
Membrane Proteins
DLG1
Oncogene Proteins, Viral
Molecular biology
Epithelium
Cell biology
DNA-Binding Proteins
030104 developmental biology
medicine.anatomical_structure
Membrane protein
Host-Pathogen Interactions
biology.protein
CIENCIAS NATURALES Y EXACTAS
Intracellular
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 97
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Journal of general virology
- Accession number :
- edsair.doi.dedup.....366516ee905e1600086a8a10472832fd