Back to Search
Start Over
Benzene metabolites induce the loss and long arm deletion of chromosomes 5 and 7 in human lymphocytes
- Source :
- Leukemia Research. 22:105-113
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- Two of the most common cytogenetic changes in therapy- and chemically-related leukemia are the loss and long (q) arm deletions of chromosomes 5 and 7 (i.e. -5, -7, del(5q) and del(7q)). We have used a novel fluorescence in situ hybridization (FISH) procedure to determine if the benzene metabolites hydroquinone (HQ) and 1,2,4-benzenetriol (BT) can induce these specific changes in human lymphocytes cultured as whole blood. Metaphase spreads were prepared and hybridized with centromeric probes for chromosomes 1, 5 and 7 and sequence specific probes for 5q31 and 7q36-qter. HQ and BT significantly increased monosomy 5 and 7 by 3-5 fold (p < 0.0001). Both HQ and BT also significantly increased the rate of del(5q) and del(7q) by 8-12 fold (p < 0.0001). Chromosome 7 was especially susceptible to aneusomy induction by HQ and BT at low doses. These results show that metabolites of benzene are highly effective in inducing changes in chromosomes 5 and 7 that are involved in the development of myeloid leukemia.
- Subjects :
- Cancer Research
medicine.medical_specialty
Aneuploidy
Biology
medicine
Humans
Lymphocytes
Metaphase
In Situ Hybridization, Fluorescence
Carcinogen
Chromosome 7 (human)
medicine.diagnostic_test
Cytogenetics
Infant
Myeloid leukemia
Hematology
medicine.disease
Molecular biology
Hydroquinones
Leukemia
Oncology
Chromosomes, Human, Pair 5
Chromosome Deletion
Chromosomes, Human, Pair 7
Mutagens
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 01452126
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Leukemia Research
- Accession number :
- edsair.doi.dedup.....3658f5f680994ab1a17429f17f8a449b