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Detection of novel piroplasmid species and Babesia microti and Theileria orientalis genotypes in hard ticks from Tengchong County, Southwest China
- Source :
- Parasitology Research. 119:1259-1269
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- To reveal the genetic diversity of Babesia microti and Theileria orientalis in Southwest China, we conducted a molecular survey of piroplasms in hard ticks in a China-Myanmar border county. Host infesting and questing ticks were collected from Tengchong County in 2013 and 2014. Piroplasm infection in ticks was detected by PCR, and then, phylogenetic analysis was conducted to study the genetic diversity of the pathogens identified in ticks. All in all, six piroplasm species comprising of B. microti; B. orientalis; a novel Babesia species designated Babesia sp. Tengchong, China; T. orientalis; T. luwenshuni; and an as yet undescribed piroplasmid species referred to as Piroplasmid sp. Tengchong, China, have been identified after screening goat- and cattle-attached ticks. In addition, B. bigemina has been identified by screening questing ticks. Phylogenetic analysis based on the 18S rRNA and partial β-tubulin gene revealed two novel potentially zoonotic genotypes designated B. microti Tengchong-Type A and B. The T. orientalis genotypes identified in the present study represent the seven known genotypes 1-5, 7, and N3 as revealed by phylogenetic analysis of 18S rRNA and MPSP genes. Importantly, an additional genotype designated N4 has also been identified in this study, which brings the number of recognized T. orientalis genotypes to a total of twelve. Thus, besides the two novel species, Babesia sp. Tengchong, China, closely related to Babesia species isolated from yak and Piroplasmid sp. Tengchong, China, our study demonstrates that additional novel B. microti and T. orientalis genotypes exist in Southwest China.
- Subjects :
- China
Genotype
Ixodidae
030231 tropical medicine
Babesia
Zoology
BABESIA MICROTI
Myanmar
Biology
Babesia microti
Polymerase Chain Reaction
18S ribosomal RNA
030308 mycology & parasitology
03 medical and health sciences
0302 clinical medicine
Theileria
parasitic diseases
RNA, Ribosomal, 18S
Animals
Phylogeny
0303 health sciences
Genetic diversity
General Veterinary
Phylogenetic tree
Host (biology)
General Medicine
Infectious Diseases
Insect Science
Theileria orientalis
Cattle
Parasitology
Hard ticks
Subjects
Details
- ISSN :
- 14321955 and 09320113
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Parasitology Research
- Accession number :
- edsair.doi.dedup.....36480aa482777bf431fad339412b2027