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Antimicrobial and antibiofilm activities of prenylated flavanones from Macaranga tanarius
- Source :
- Phytomedicine : international journal of phytotherapy and phytopharmacology. 63
- Publication Year :
- 2019
-
Abstract
- Background The emergence of antibiotic resistant microorganisms presents a worldwide problem that requires novel antibiotic and non-antibiotic strategies, and biofilm formation is a mechanism of drug resistance utilized by diverse microorganisms. The majority of microorganisms live in biofilms that help their survival against starvation, antimicrobial agents, and immunological defense systems. Therefore, it is important novel compounds be identified that inhibit biofilm formation and cell survival without drug resistance. Study design In this study, the antimicrobial and antibiofilm activities of five prenylated flavanones (Okinawan propolins) isolated from fruits of Macaranga tanarius (L.) were investigated against 14 microorganisms including 10 pathogens. Results Of these five propolins, propolin D at 5–10 µg/ml significantly inhibited biofilm formation by three Staphylococcus aureus strains, a Staphylococcus epidermidis strain, and a Candida albicans with MICs from 10 to 50 µg/ml, and in C. albicans, propolin D was found to inhibit biofilm formation by reducing cell aggregation and downregulated the expressions of hypha/biofilm-related genes including ECE1 and HWP1. Interestingly, at sub-MIC concentrations (10–50 µg/ml), propolin D significantly inhibited biofilm formation by enterohemorrhagic E. coli O157:H7, uropathogenic E. coli O6:H1, and Acinetobacter baumannii without affecting planktonic cell growth, but did not inhibit biofilm formation by a commensal E. coli K-12 strain, three probiotic Lactobacillus plantarum strains, or two Pseudomonas aeruginosa strains. And, propolin D reduced fimbriae production by E. coli O157:H7 and repressed gene expression of curli fimbriae genes (csgA and csgB). Also, propolin D was minimally toxic in a Caenorhabditis elegans nematode model. Conclusion These findings show that prenylated flavanones, especially propolin D from Macaranga tanarius (Okinawan propolis), should be considered potential candidates for the development of non-toxic antibacterial and antifungal agents against persistent microorganisms.
- Subjects :
- Staphylococcus aureus
Antifungal Agents
Drug Evaluation, Preclinical
Pharmaceutical Science
Drug resistance
Microbial Sensitivity Tests
medicine.disease_cause
Escherichia coli O157
Microbiology
Macaranga tanarius
03 medical and health sciences
0302 clinical medicine
Staphylococcus epidermidis
Drug Discovery
Candida albicans
Toxicity Tests
medicine
Animals
Caenorhabditis elegans
030304 developmental biology
Pharmacology
Flavonoids
Prenylation
0303 health sciences
biology
Pseudomonas aeruginosa
Chemistry
Biofilm
Euphorbiaceae
Antimicrobial
biology.organism_classification
Cell aggregation
Anti-Bacterial Agents
Complementary and alternative medicine
030220 oncology & carcinogenesis
Biofilms
Flavanones
Molecular Medicine
Subjects
Details
- ISSN :
- 1618095X
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Phytomedicine : international journal of phytotherapy and phytopharmacology
- Accession number :
- edsair.doi.dedup.....36402c4473703410a5db5911256b290d