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Some characteristics of a cellular receptor for virulent infectious bursal disease virus by using flow cytometry
- Source :
- Archives of Virology. 143:2327-2341
- Publication Year :
- 1998
- Publisher :
- Springer Science and Business Media LLC, 1998.
-
Abstract
- A flow cytometric virus binding assay that directly visualizes the binding of infectious bursal disease virus (IBDV) to its target cells was established. The chicken B lymphoblastoid cell line, LSCC-BK3, which is permissive for IBDV infection, bound high levels of the virus. Another B lymphoblastoid cell line, LSCC-1104-B1, bound low levels of the virus, although it was nonpermissive. No virus binding was detected in nonpermissive T lymphoblastoid cell lines. In the binding assay to heterogeneous cell populations of chicken lymphocytes, IBDV (a highly virulent OKYM strain) bound to 94% cells in the lymphocytes prepared from the bursa of Fabricius, 37% cells in those prepared from the spleen, 3% cells in those prepared from the thymus, and 21% cells in those prepared from the blood. Most of the cells, which bound the virus, were surface immunoglobulin M (SIgM)-positive, but a small number of them were SIgM-negative. Additionally, the binding of IBDV to the LSCC-BK3 cells was affected by treatment of the cells with proteases and N-glycosylation inhibitors. These findings may indicate that the IBDV host range is mainly controlled by the presence of a virus receptor composed of N-glycosylated protein associated with the subtle differentiation stage of B-lymphocytes represented mostly by SIgM-bearing cells.
- Subjects :
- Glycosylation
animal structures
Birnaviridae
Biotin
Receptors, Antigen, B-Cell
Virulence
Spleen
Biology
Infectious bursal disease virus
Virus
Cell Line
Infectious bursal disease
Flow cytometry
Virology
Endopeptidases
medicine
Animals
Bursa of Fabricius
Poultry Diseases
B-Lymphocytes
medicine.diagnostic_test
Virus receptor
General Medicine
Birnaviridae Infections
Flow Cytometry
medicine.disease
biology.organism_classification
medicine.anatomical_structure
Immunoglobulin M
Receptors, Virus
Chickens
Subjects
Details
- ISSN :
- 14328798 and 03048608
- Volume :
- 143
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....362b52e7d2fa0de720c2f34f576daa98