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Phenotypic and Genotypic Antimicrobial Resistance Traits of Vibrio cholerae Non-O1/Non-O139 Isolated From a Large Austrian Lake Frequently Associated With Cases of Human Infection
- Source :
- Frontiers in Microbiology, Frontiers in Microbiology, Frontiers Media, 2019, 10 (2600), pp.1-9. ⟨10.3389/fmicb.2019.02600⟩, Frontiers in Microbiology, Vol 10 (2019)
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- International audience; Vibrio cholerae belonging to serogroups other than O1 and O139 are opportunistic pathogens which cause infections with a variety of clinical symptoms. Due to the increasing number of V. cholerae non-O1/non-O139 infections in association with recreational waters in the past two decades, they have received increasing attention in recent literature and by public health authorities. Since the treatment of choice is the administration of antibiotics, we investigated the distribution of antimicrobial resistance properties in a V. cholerae non-O1/non-O139 population in a large Austrian lake intensively used for recreation and in epidemiologically linked clinical isolates. In total, 82 environmental isolates-selected on the basis of comprehensive phylogenetic information-and nine clinical isolates were analyzed for their phenotypic antimicrobial susceptibility. The genomes of 46 environmental and eight clinical strains were screened for known genetic antimicrobial resistance traits in CARD and ResFinder databases. In general, antimicrobial susceptibility of the investigated V. cholerae population was high. The environmental strains were susceptible against most of the 16 tested antibiotics, except sulfonamides (97.5% resistant strains), streptomycin (39% resistant) and ampicillin (20.7% resistant). Clinical isolates partly showed additional resistance to amoxicillin-clavulanic acid. Genome analysis showed that crp, a regulator of multidrug efflux genes, and the bicyclomycin/multidrug efflux system of V. cholerae were present in all isolates. Nine isolates additionally carried variants of bla CARB−7 and bla CARB−9 , determinants of beta-lactam resistance and six isolates carried catB9, a determinant of phenicol resistance. Three isolates had both bla CARB−7 and catB9. In 27 isolates, five out of six subfamilies of the MATE-family were present. For all isolates no genes conferring resistance to aminoglycosides, macrolides and sulfonamides were detected. The apparent lack of either known antimicrobial resistance traits or mobile genetic elements indicates that in cholera non-epidemic regions of the world, V. cholerae non-O1/non-O139 play a minor role as a reservoir of resistance in the environment. The discrepancies The discrepancies between the phenotypic and genome-based antimicrobial resistance assessment show that for V. cholerae non-O1/non-O139, resistance databases are currently inappropriate for an assessment of antimicrobial resistance. Continuous collection of both data overtime may solve such discrepancies between genotype and phenotype in the future between the phenotypic and genome-based antimicrobial resistance assessment showthat for V. cholerae non-O1/non-O139, resistance databases are currently inappropriatefor an assessment of antimicrobial resistance. Continuous collection of both data overtime may solve such discrepancies between genotype and phenotype in the future.
- Subjects :
- Microbiology (medical)
lac
antibiotic resistance
medicine.drug_class
phenotype
genotype
Antibiotics
Population
water
lcsh:QR1-502
Drug resistance
Biology
medicine.disease_cause
human health
lcsh:Microbiology
Microbiology
Bicyclomycin
03 medical and health sciences
Antibiotic resistance
résistance aux antimicrobiens
Vibrio cholerae non-O1
medicine
eau de baignade
antimicrobial resistance
microbiologie
education
bacteria
lake
Vibrio cholerae
030304 developmental biology
bactérie
0303 health sciences
Antiinfective agent
education.field_of_study
030306 microbiology
bathing water
microbiology
transmission
non-O1/non-O139
homme
3. Good health
phénotype
climate change
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
génotype
Subjects
Details
- Language :
- English
- ISSN :
- 1664302X
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology, Frontiers in Microbiology, Frontiers Media, 2019, 10 (2600), pp.1-9. ⟨10.3389/fmicb.2019.02600⟩, Frontiers in Microbiology, Vol 10 (2019)
- Accession number :
- edsair.doi.dedup.....3624dc9d375e9ed841151b4409c3faf7