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Behavioral abnormalities in female mice following administration of aluminum adjuvants and the human papillomavirus (HPV) vaccine Gardasil
Behavioral abnormalities in female mice following administration of aluminum adjuvants and the human papillomavirus (HPV) vaccine Gardasil
- Source :
- Repositorio EdocUR-U. Rosario, Universidad del Rosario, instacron:Universidad del Rosario
- Publication Year :
- 2017
- Publisher :
- Humana Press Inc., 2017.
-
Abstract
- Vaccine adjuvants and vaccines may induce autoimmune and inflammatory manifestations in susceptible individuals. To date most human vaccine trials utilize aluminum (Al) adjuvants as placebos despite much evidence showing that Al in vaccine-relevant exposures can be toxic to humans and animals. We sought to evaluate the effects of Al adjuvant and the HPV vaccine Gardasil versus the true placebo on behavioral and inflammatory parameters in female mice. Six-week-old C57BL/6 female mice were injected with either, Gardasil, Gardasil + pertussis toxin (Pt), Al hydroxide, or, vehicle control in amounts equivalent to human exposure. At 7.5 months of age, Gardasil and Al-injected mice spent significantly more time floating in the forced swimming test (FST) in comparison with vehicle-injected mice (Al, p = 0.009; Gardasil, p = 0.025; Gardasil + Pt, p = 0.005). The increase in floating time was already highly significant at 4.5 months of age for the Gardasil and Gardasil + Pt group (p ? 0.0001). No significant differences were observed in the number of stairs climbed in the staircase test which measures locomotor activity. These results indicate that differences observed in the FST were unlikely due to locomotor dysfunction, but rather due to depression. Moreover, anti-HPV antibodies from the sera of Gardasil and Gardasil + Pt-injected mice showed cross-reactivity with the mouse brain protein extract. Immunohistochemistry analysis revealed microglial activation in the CA1 area of the hippocampus of Gardasil-injected mice. It appears that Gardasil via its Al adjuvant and HPV antigens has the ability to trigger neuroinflammation and autoimmune reactions, further leading to behavioral changes. © 2016, Springer Science+Business Media New York.
- Subjects :
- Allergy
Mouse
medicine.medical_treatment
Enzyme linked immunosorbent assay
Immunological adjuvant
Aluminum Hydroxide
Autoimmunity
Pharmaceutical vehicles and additives
Antibodies, Viral
medicine.disease_cause
Hippocampus
Animal tissue
Hpv l1 protein
Mice
0302 clinical medicine
Autoantibody
Cognition
Human Papillomavirus Recombinant Vaccine Quadrivalent, Types 6, 11, 16, 18
Brain perfusion
Neuroinflammation
Pertussis toxin
Virus antibody
Human papillomavirus recombinant vaccine quadrivalent, types 6, 11, 16, 18
Behavior disorder
animal
immunologic
human papillomavirus
Antigens, Viral
Priority journal
Behavior, Animal
biology
Oncogene proteins
Immunohistochemistry
Staircase test
Virus antigen
Blood
pharmaceutic
Female
Forced swim test
Microglia
Antibody
inbred c57bl
Adjuvant
Locomotion
Animal behavior
medicine.drug
viral
Capsid protein
Immunology
Oncoprotein
Article
Antibodies
03 medical and health sciences
Gardasil
Adjuvants, Immunologic
Antigen
medicine
C57bl mouse
Animals
Animal model
Asia syndrome
Adjuvants
Antigens
Swimming
Adjuvants, Pharmaceutic
Autoantibodies
030203 arthritis & rheumatology
Behavior
Drug effects
business.industry
Recognition, Psychology
Oncogene Proteins, Viral
medicine.disease
Nonhuman
Mice, Inbred C57BL
Recognition
Capsid proteins
Enzyme inhibition assay
biology.protein
Capsid Proteins
Aluminum hydroxide
Visual memory
business
Wart virus vaccine
Controlled study
Recognition (psychology)
030217 neurology & neurosurgery
Behavioural despair test
Aluminum
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Repositorio EdocUR-U. Rosario, Universidad del Rosario, instacron:Universidad del Rosario
- Accession number :
- edsair.doi.dedup.....35f2656ec021069b369c4c2f74f6a35d