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Identification, Isolation, and Functional Assay of Regulatory T Cells
- Source :
- Immunological Investigations. 45:584-602
- Publication Year :
- 2016
- Publisher :
- Informa UK Limited, 2016.
-
Abstract
- Two categories of regulatory T cells (Tregs), nTreg and iTreg, play vital roles in orchestrating the integrity of a host in the course of an immune response. Tregs commonly belong to CD4+ CD25+ T cells and they are characterized by a transcription factor - forkhead box P3 (FoxP3). Within the space of the last few years, interests have been drawn to Tregs as a therapeutic tool in several settings like autoimmune disease, transplantation, and tumor disorders. As a consequence, to assess the functional properties of Tregs, namely through their ability to suppress other cells, cytokine expression, and proliferation in a variety of conditions, it is mandatory to gain better approaches to this end. This would be beneficial in designing better-than-ever therapeutic methods with regard to Tregs properties. Gaining better insights into the underlying mechanisms of immune regulation, by means of straightforward and less time-consuming techniques, will hopefully permit the therapeutic application of these cells in the control of human disorders. This review aims at going through the basic methods for Treg isolation as well the efficiency of the commonly exerted in vitro assays of Tregs-mediated immune suppression.
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
Immunology
chemical and pharmacologic phenomena
Biology
T-Lymphocytes, Regulatory
Immune tolerance
03 medical and health sciences
0302 clinical medicine
Immune system
T-Lymphocyte Subsets
Immune Tolerance
medicine
Animals
Humans
IL-2 receptor
Immunoassay
Autoimmune disease
Interleukin-2 Receptor alpha Subunit
In vitro toxicology
FOXP3
Forkhead Transcription Factors
hemic and immune systems
General Medicine
Immunotherapy
medicine.disease
Transplantation
030104 developmental biology
CD4 Antigens
030215 immunology
Subjects
Details
- ISSN :
- 15324311 and 08820139
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Immunological Investigations
- Accession number :
- edsair.doi.dedup.....35eda614bf885b246f9bdcd861775f30