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Correlation between arrested secondary plasmodesmal development and onset of accelerated leaf senescence in yeast acid invertase transgenic tobacco plants
- Source :
- The Plant journal : for cell and molecular biology. 4(1)
- Publication Year :
- 1993
-
Abstract
- Mature leaves of a transgenic tobacco plant (Nicotiana tabacum L var. Samsun, line A41-10) that constitutively express a yeast-derived acid invertase gene develop symptoms which are characterized by the presence of greenish-yellow and green sectors in the same leaf, and onset of early, leaf senescence. Previous studies indicated that invertase activity was two- to threefold higher in the greenish-yellow sectors than in the green sectors. Our structural analyses revealed that development of secondary plasmodesmata, via modification of existing primary plasmodesmata, between mesophyll cells was inhibited severely in the greenish-yellow sectors, but only marginally in the green sectors. In contrast, the structure and function of primary plasmodesmata in the same symptomatic sectors remained unaltered as determined by structural and dye coupling studies. It is hypothesized that secondary plasmodesmata differ from primary plasmodesmata in having special abilities to traffic information molecules to coordinate leaf development and physiological function(s). Arrest of secondary plasmodesmal development by high invertase activity in the transgenic tobacco leaf may have prevented this type of trafficking and hence resulted in early leaf senescence. The results also indicate that the yeast acid invertase-expressing tobacco may provide an effective experimental system for the molecular characterization of cellular mechanisms that regulate the development, function, and possible turnover of secondary plasmodesmata.
- Subjects :
- Senescence
Polymorphism, Genetic
Glycoside Hydrolases
beta-Fructofuranosidase
Transgene
Nicotiana tabacum
fungi
food and beverages
Gene Expression
Cell Biology
Plant Science
Plasmodesma
Saccharomyces cerevisiae
Biology
biology.organism_classification
Plants, Genetically Modified
Yeast
Microscopy, Electron
Plants, Toxic
Invertase
Biochemistry
Tobacco
Genetics
Gene
Function (biology)
Subjects
Details
- ISSN :
- 09607412
- Volume :
- 4
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biology
- Accession number :
- edsair.doi.dedup.....35ed0579d2c587399c1db0fab28a28da