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Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway
- Source :
- Journal of immunology (Baltimore, Md. : 1950). 195(8)
- Publication Year :
- 2015
-
Abstract
- Aspirin-exacerbated respiratory disease (AERD), a severe eosinophilic inflammatory disorder of the airways, involves overproduction of cysteinyl leukotrienes (cysLTs), activation of airway mast cells (MCs), and bronchoconstriction in response to nonselective cyclooxygenase inhibitors that deplete homeostatic PGE2. The mechanistic basis for MC activation in this disorder is unknown. We now demonstrate that patients with AERD have markedly increased epithelial expression of the alarmin-like cytokine IL-33 in nasal polyps, as compared with polyps from aspirin-tolerant control subjects. The murine model of AERD, generated by dust mite priming of mice lacking microsomal PGE2 synthase (ptges−/− mice), shows a similar upregulation of IL-33 protein in the airway epithelium, along with marked eosinophilic bronchovascular inflammation. Deletion of leukotriene C4 synthase, the terminal enzyme needed to generate cysLTs, eliminates the increased IL-33 content of the ptges−/− lungs and sharply reduces pulmonary eosinophilia and basal secretion of MC products. Challenges of dust mite–primed ptges−/− mice with lysine aspirin induce IL-33–dependent MC activation and bronchoconstriction. Thus, IL-33 is a component of a cysLT-driven innate type 2 immune response that drives pathogenic MC activation and contributes substantially to AERD pathogenesis.
- Subjects :
- Adult
Male
Leukotrienes
Adolescent
medicine.medical_treatment
Immunology
Inflammation
Biology
Dinoprostone
Article
Type 2 immune response
chemistry.chemical_compound
Mice
medicine
Immunology and Allergy
Animals
Humans
Mast Cells
Pulmonary Eosinophilia
Aged
Prostaglandin-E Synthases
Mice, Knockout
Leukotriene C4
respiratory system
Middle Aged
Interleukin-33
Immunity, Innate
respiratory tract diseases
Interleukin 33
Intramolecular Oxidoreductases
Cytokine
chemistry
Respiratory epithelium
Bronchoconstriction
Asthma, Aspirin-Induced
Female
medicine.symptom
Subjects
Details
- ISSN :
- 15506606
- Volume :
- 195
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Accession number :
- edsair.doi.dedup.....35e70d555e1930b8335dbf099f6c4e3b