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Accumulation of 2'-O-Methylguanosine Deficient tRNATrp in Tryptophan Limited Saccharomyces cerevisiae
- Source :
- Microbiology. 128:2591-2600
- Publication Year :
- 1982
- Publisher :
- Microbiology Society, 1982.
-
Abstract
- SUMMARY: Saccharomyces cerevisiae synthesizes one major tryptophan transfer ribonucleic acid (tRNATrp) species (isoacceptor A) carrying characteristic base modifications. Under tryptophan limited growth conditions wild-type strain X2180-1A exhibited a second important tRNATrp species (isoacceptor B). The total amount of tRNATrp, approximately 2.5 pmol (mg dry wt)-1, stayed essentially constant during amino acid shift-down experiments. The amount of isoacceptor B relative to total tRNATrp was 10 to 15% during amino acid sufficient exponential growth conditions, but increased during tryptophan limitation three- to four-fold. Analysis of the base compositions showed that isoacceptor B differed from isoacceptor A in one respect only: 2′-O-methylguanosine, a modified guanosine base occurring at position 17 of the major isoacceptor A tRNATrp, was not detectable in hydrolysates of purified isoacceptor B. The biological significance of isoacceptor B is discussed.
- Subjects :
- chemistry.chemical_classification
Guanosine
biology
Strain (chemistry)
Saccharomyces cerevisiae
Tryptophan
Nucleosides
biology.organism_classification
Microbiology
Hydrolysate
Amino acid
chemistry.chemical_compound
Phenotype
RNA, Transfer
chemistry
Biochemistry
Biological significance
Mutation
Transfer RNA
Subjects
Details
- ISSN :
- 14652080 and 13500872
- Volume :
- 128
- Database :
- OpenAIRE
- Journal :
- Microbiology
- Accession number :
- edsair.doi.dedup.....35d7417eadc8cd931f326ffe187bb14b