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Altered mucosal DNA methylation in parallel with highly active Helicobacter pylori-related gastritis
- Source :
- Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association. 16(4)
- Publication Year :
- 2012
-
Abstract
- Chronic inflammation triggered by Helicobacter pylori causes altered DNA methylation in stomach mucosae, which is deeply involved in gastric carcinogenesis. This study aimed to elucidate the correlation between altered mucosal DNA methylation levels and activity of H. pylori-related gastritis, because inflammatory activity shows particular correlations with the development of diffuse-type cancer.Methylation levels in stomach mucosae of 78 healthy volunteers were determined by real-time methylation-specific PCR or bisulfite pyrosequencing. Examined loci were the promoter CpG islands of six genes (FLNc, HAND1, THBD, p41ARC, HRASLS, and LOX) and the CpG sites of non-coding repetitive elements (Alu and SatĪ±) that are reportedly altered by H. pylori infection. Activity of H. pylori-related gastritis was evaluated using two serum markers: H. pylori antibody titer and pepsinogen II.Methylation levels of the six CpG islands were consistently increased, and those of the two repetitive elements were consistently decreased in a stepwise manner with the activity of gastric inflammation as represented by serum marker levels. Each serum marker level was well correlated with the overall DNA methylation status of stomach mucosa, and these two serologic markers were additive in the detection of the mucosa with severely altered DNA methylation.Alteration in mucosal DNA methylation level was closely correlated with activity of H. pylori-related gastritis as evaluated by serum markers. The observed correlation between altered DNA methylation levels and activity of H. pylori-related gastritis appears to be one of the relevant molecular mechanisms underlying the development of diffuse-type cancer.
- Subjects :
- Male
Cancer Research
Thrombomodulin
medicine.disease_cause
Basic Helix-Loop-Helix Transcription Factors
Medicine
Promoter Regions, Genetic
Aged, 80 and over
biology
Stomach
Gastroenterology
General Medicine
Middle Aged
Prognosis
Scavenger Receptors, Class E
medicine.anatomical_structure
Oncology
CpG site
Gastritis
DNA methylation
Female
medicine.symptom
Adult
Filamins
Inflammation
Actin-Related Protein 2-3 Complex
Phospholipases A
Helicobacter Infections
Young Adult
Stomach Neoplasms
Humans
Aged
Helicobacter pylori
business.industry
Cancer
Proteins
DNA Methylation
biology.organism_classification
medicine.disease
Gastric Mucosa
Case-Control Studies
Immunoglobulin G
Cancer research
CpG Islands
business
Carcinogenesis
Biomarkers
Follow-Up Studies
Subjects
Details
- ISSN :
- 14363305
- Volume :
- 16
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association
- Accession number :
- edsair.doi.dedup.....35d6f0b8a6d331b7f5551c7068e33982