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Cloning, sequencing and expression in the dog of the main amyloid precursor protein isoforms and some of the enzymes related with their processing
- Source :
- Neuroscience. 171(4)
- Publication Year :
- 2010
-
Abstract
- Alzheimer's disease (AD) is characterized by neuronal loss and the presence of both neurofibrillary tangles and senile plaques in the brain. These plaques arise from the deposition of beta-amyloid (Aβ) peptides (38-43 amino acids), which are generated from enzymatic cleavage of the amyloid precursor protein (APP) by β- and γ-secretases. In the present work, we cloned the principal APP isoforms as well as some enzymes that have been implicated in their amyloidogenic and non-amyloidogenic processing in dogs. Additionally, the main proteases implicated in the degradation of Aβ were also studied. We also investigated the level of expression of these APP isoforms and enzymes in different brain regions and in peripheral tissues. Our data demonstrate that these canine proteins are highly homologous to their human counterparts. In addition, the expression pattern of these proteins in dogs is consistent with previous data reported in human beings. Thus, dogs may be a natural model to study the biology of AD and could also serve as an animal model for Aβ-targeted drugs against this devastating disease.
- Subjects :
- Gene isoform
Male
medicine.medical_specialty
Proteases
Molecular Sequence Data
Gene Expression
Biology
Presenilin
Amyloid beta-Protein Precursor
Apolipoproteins E
Dogs
Internal medicine
medicine
Amyloid precursor protein
Animals
Aspartic Acid Endopeptidases
Humans
Protein Isoforms
Senile plaques
Cloning, Molecular
chemistry.chemical_classification
General Neuroscience
P3 peptide
Presenilins
Brain
Sequence Analysis, DNA
medicine.disease
Enzyme
Endocrinology
chemistry
Biochemistry
biology.protein
Female
Alzheimer's disease
Amyloid Precursor Protein Secretases
Sequence Alignment
Subjects
Details
- ISSN :
- 18737544
- Volume :
- 171
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Neuroscience
- Accession number :
- edsair.doi.dedup.....35c6e67ab7526e1a2c21bd6420fb6c58