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Protein Binding of a Novel Platinum-Based Anticancer Agent BP-C1 Containing a Lignin-Derived Polymeric Ligand
- Source :
- Applied Sciences, Vol 11, Iss 11008, p 11008 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Platinum (Pt) antineoplastic agents remain indispensable for the treatment of oncological disease. Pt-based drugs are mainly used in the therapy of ovarian cancer and non-small-cell lung carcinoma. A novel platinum-containing antineoplastic agent BP-C1 is a complex of diamminoplatinum with an oxygen-donor polymeric ligand of benzene-polycarboxylic acids, isolated from natural lignin. The aim of the study was to investigate ex vivo protein binding of BP-C1. Protein binding of BP-C1 was tested using equilibrium dialysis. Pooled blood plasma was used in the study. Control solutions contained the same dosages of BP-C1 in PBS (pH 7.2). Plasma and control solutions were submitted to equilibrium dialysis across a vertical 8 kDa cut-off membrane for 4 h at 37 °C under gentle shaking. Platinum was quantified in dialysis and retained fractions using inductively coupled plasma mass spectrometry after microwave digestion. The dialysis system was tested and validated; this showed no protein saturation with platinum. A medium degree of binding of platinum to macromolecular species of ca. 60% was observed. The study showed the maintenance of a high fraction of free BP-C1 in the bloodstream, facilitating its pharmacological activity.
- Subjects :
- Technology
QH301-705.5
QC1-999
lignin
chemistry.chemical_element
protein binding
Plasma protein binding
General Materials Science
Equilibrium dialysis
Biology (General)
equilibrium dialysis
inductively coupled plasma mass spectrometry
QD1-999
Instrumentation
Inductively coupled plasma mass spectrometry
Fluid Flow and Transfer Processes
Chromatography
Physics
Process Chemistry and Technology
General Engineering
Engineering (General). Civil engineering (General)
Ligand (biochemistry)
Computer Science Applications
anticancer drugs
Chemistry
Membrane
chemistry
free platinum
TA1-2040
Platinum
Dialysis (biochemistry)
Ex vivo
Subjects
Details
- ISSN :
- 20763417
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Applied Sciences
- Accession number :
- edsair.doi.dedup.....35c4d55ea8911bb703347898e23f30a7