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Synthesis of radioactive dolichol from [4S-3H]-mevalonate in the regenerating rat liver
- Source :
- Biochemical Journal. 138:277-280
- Publication Year :
- 1974
- Publisher :
- Portland Press Ltd., 1974.
-
Abstract
- The dolichol of rat liver was labelled by injecting [4S-3H]mevalonate, the precursor of cis-isoprene residues, into partially hepatectomized animals. The optimum conditions for labelling the dolichol were to inject the animals with radioactive mevalonate 48h after hepatectomy and to kill them 12h later. The concentration of radioactive dolichol was five times as great in regenerating rat liver as in normal liver. The highest concentration of radioactive dolichol was found in the crude mitochondrial and nuclear-debris fractions of the cell. The crude microsomal fractions also contained radioactive dolichol, but at a lower concentration.
- Subjects :
- Male
History
Time Factors
medicine.medical_treatment
Cell
Mevalonic Acid
Mitochondria, Liver
Tritium
Education
chemistry.chemical_compound
Cytosol
Dolichol
Labelling
medicine
Animals
Hepatectomy
Chromatography
Chemistry
Biosynthesis and Degradation
Liver Regeneration
Rats
Computer Science Applications
medicine.anatomical_structure
Liver
Biochemistry
Rat liver
Microsomes, Liver
Microsome
Female
lipids (amino acids, peptides, and proteins)
Chromatography, Thin Layer
Diterpenes
Fatty Alcohols
Subcellular Fractions
Subjects
Details
- ISSN :
- 03063283
- Volume :
- 138
- Database :
- OpenAIRE
- Journal :
- Biochemical Journal
- Accession number :
- edsair.doi.dedup.....35baa62e9de943d1cc3209d268d09ae4