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Purification, characterization and in vitro antioxidant activities of polysaccharide from Chaenomeles speciosa
- Source :
- International Journal of Biological Macromolecules. 92:702-707
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- A water-soluble polysaccharide named as CSP-2 was isolated and purified from Chaenomeles speciosa by DEAE-Sepharose and Sephadex G-100 column chromatography. CSP-2 was composed of galactose (Gal), rhamnose (Rha), glucose (Glc) and xylose (Xyl) with a relative molar ratio of 3.8:1.6:1.2:0.4. The average molecular weight of CSP-2 was estimated to be about 4.6×104Da. The in vitro antioxidant assay showed that CSP-2 exhibited remarkable antioxidant activities to scavenge the DPPH radical, superoxide radical, hydroxyl radical and ABTS radical and significant cytoprotective effects on H2O2-induced PC12 cells injury in a concentration-dependent manner. Overall, CSP-2 may be useful as a naturally potential antioxidant agent for application in food and medicinal fields.
- Subjects :
- Antioxidant
DPPH
medicine.medical_treatment
02 engineering and technology
PC12 Cells
Rhamnose
01 natural sciences
Biochemistry
chemistry.chemical_compound
Superoxides
Structural Biology
Chaenomeles speciosa
chemistry.chemical_classification
Xylose
ABTS
biology
Free Radical Scavengers
General Medicine
Chromatography, Ion Exchange
021001 nanoscience & nanotechnology
Chromatography, Gel
0210 nano-technology
Cell Survival
Polysaccharide
Picrates
Polysaccharides
parasitic diseases
medicine
Animals
Benzothiazoles
Rosaceae
Molecular Biology
Chromatography
Hydroxyl Radical
Plant Extracts
010405 organic chemistry
Biphenyl Compounds
Galactose
Water
Hydrogen Peroxide
biology.organism_classification
Rats
0104 chemical sciences
Molecular Weight
Glucose
Solubility
chemistry
Hydroxyl radical
Sulfonic Acids
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....35b991a53de56a69a87b94b8d493d6d1