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Hemorrhagic shock alters fracture callus composition and activates the IL6 and RANKL/OPG pathway in mice
- Source :
- Journal of Trauma and Acute Care Surgery. 85:359-366
- Publication Year :
- 2018
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2018.
-
Abstract
- Background Fracture and hemorrhagic shock often lead to impaired fracture healing. To elucidate underlying pathogenesis, this study aimed to analyze histological properties during fracture healing after hemorrhagic shock and involved signaling pathways in mice. Methods Male C57BL/6NCrl mice were assigned into five groups. Control group underwent no interventions. Sham group had a catheter and external fixator but neither blood loss nor osteotomy. Trauma-hemorrhage (TH) group received a pressure-controlled hemorrhagic shock; osteotomy (Fx) group, an osteotomy and fixator; and combined trauma (THFx) group, both hemorrhagic shock and externally fixed osteotomy. After 1, 2, 3, and 4 weeks, the animals were killed. Undecalcified bones were analyzed histologically and signaling pathways relevant for fracture healing by polymerase chain reaction and Western blot. Statistical significance was set at 0.05 or less. Comparisons were performed using the Mann-Whitney U or Kruskal-Wallis test. Results In the THFx group, a decreased bone formation after 3 weeks, a reduction of both bone and cartilage after 2 weeks, and an enhanced activation of the RANKL/OPG and IL6 signaling pathway after 1 week were shown in comparison to Fx. Conclusions Hemorrhagic shock has a retarding effect on fracture healing in the early phase of fracture healing and leads to activation of the IL6 and RANKL/OPG signaling pathways.
- Subjects :
- Male
0301 basic medicine
Pathology
medicine.medical_specialty
medicine.medical_treatment
Bone healing
Shock, Hemorrhagic
Critical Care and Intensive Care Medicine
Osteotomy
Pathogenesis
Fractures, Bone
Mice
03 medical and health sciences
0302 clinical medicine
Western blot
Animals
Medicine
Bony Callus
Survival rate
Fracture Healing
medicine.diagnostic_test
biology
Interleukin-6
business.industry
Cartilage
RANK Ligand
Osteoprotegerin
Mice, Inbred C57BL
Survival Rate
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
RANKL
030220 oncology & carcinogenesis
Shock (circulatory)
biology.protein
Surgery
medicine.symptom
business
Signal Transduction
Subjects
Details
- ISSN :
- 21630763 and 21630755
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Journal of Trauma and Acute Care Surgery
- Accession number :
- edsair.doi.dedup.....35ae8b55da2f39cdd0d5e69cbaf261bb