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Production of monoclonal antibodies directed to Hanganutziu-Deicher active gangliosides, N-glycolylneuraminic acid-containing gangliosides
- Source :
- Journal of biochemistry. 117(5)
- Publication Year :
- 1995
-
Abstract
- We have established three kinds of monoclonal antibodies against gangliosides containing N-glycolylneuraminic acid (NeuGc) by immunization of BALB/c mice with the purified gangliosides inserted into liposomes comprising Salmonella minnesota R595 lipopolysaccharides, and fusion of spleen cells with a mouse myeloma cell line. One monoclonal antibody, SHS-1, which was generated by immunizing mice with purified i-active ganglioside(NeuGc), reacted specifically with the i-active ganglioside(NeuGc) used as an immunogen. Structurally related gangliosides, such as GM3(NeuGc), sialosylparagloboside (SPG) (NeuGc), or I-active ganglioside(NeuGc), corresponding gangliosides [GM3 containing N-acetylneuraminic acid (NeuAc), SPG(NeuAc), i-active ganglioside(NeuAc), and I-active ganglioside(NeuAc)], other gangliosides, or neutral glycosphingolipid (GSL) were not recognized by the monoclonal antibody. These findings indicate that the SHS-1 monoclonal antibody may be specific for NeuGc-containing i-active ganglioside. On the other hand, the other two monoclonal antibodies, MSG-1 and SPS-20, which were generated by immunizing mice with purified ganglioside GM3(NeuGc) and SPG(NeuGc), respectively, showed cross-reactivity to structurally related gangliosides. The MSG-1 monoclonal antibody exhibited reactivity to ganglioside GM3(NeuAc). The SPS-20 monoclonal antibody also cross-reacted with SPG(NeuAc), i-active ganglioside(NeuGc), and i-active ganglioside(NeuAc). Neither MSG-1 nor SPS-20 reacted with corresponding gangliosides, other gangliosides, or neutral GSLs tested. Using the SHS-1 antibody specific for i-active ganglioside(NeuGc), we studied the expression of NeuGc-containing antigen in human colon cancer tissue. An NeuGc-containing glycoconjugate was detected in the colon cancer tissue.
- Subjects :
- Immunogen
Glycoconjugate
medicine.drug_class
Molecular Sequence Data
Spleen
Antibodies, Heterophile
Monoclonal antibody
Biochemistry
chemistry.chemical_compound
Mice
Antigen
N-Glycolylneuraminic acid
Antibody Specificity
Antigens, Neoplasm
Antigens, Heterophile
Gangliosides
medicine
Animals
Humans
Molecular Biology
chemistry.chemical_classification
Mice, Inbred BALB C
Ganglioside
biology
Globosides
food and beverages
Antibodies, Monoclonal
General Medicine
Molecular biology
Immunohistochemistry
medicine.anatomical_structure
chemistry
Carbohydrate Sequence
Immunology
Antibody Formation
Colonic Neoplasms
Liposomes
biology.protein
lipids (amino acids, peptides, and proteins)
Neuraminic Acids
Antibody
Subjects
Details
- ISSN :
- 0021924X
- Volume :
- 117
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of biochemistry
- Accession number :
- edsair.doi.dedup.....3597f91de5dcf1ecd51893bb1ecb6880