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Conferral of transposable properties to a chromosomal gene in Escherichia coli
- Source :
- Journal of Molecular Biology. 141:235-248
- Publication Year :
- 1980
- Publisher :
- Elsevier BV, 1980.
-
Abstract
- A normally stable gene of Escherichia coli was converted into a transposable element. A bacterial strain was constructed in which the malK gene was flanked on each side by the transposable element Tn5. The resulting Tn5-malK+-Tn5 structure (Tn651) became a transposable element with properties very similar to those of Tn5 itself. Tn651 transposes into regions of both the E. coli chromosome and bacteriophage lambda and is able to induce mutations. Transposition of Tn651 does not require the product of recA. Based on a physical analysis of lambda Tn651 DNA it is shown that the two Tn5s flanking the malK gene are in inverted orientation. In these experiments a new derivative of bacteriophage lambda is used that can accept a 14 kilobase insertion in vivo and still yield a plaque-forming transducing particle.
- Subjects :
- Transposable element
medicine.disease_cause
Lambda
Bacteriophage
Transposition (music)
chemistry.chemical_compound
Transduction, Genetic
Structural Biology
Escherichia coli
medicine
Molecular Biology
Gene
Genetics
biology
Nucleic Acid Heteroduplexes
Chromosome Mapping
Chromosome
Drug Resistance, Microbial
Chromosomes, Bacterial
biology.organism_classification
Bacteriophage lambda
Molecular biology
chemistry
DNA Transposable Elements
bacteria
DNA
Subjects
Details
- ISSN :
- 00222836
- Volume :
- 141
- Database :
- OpenAIRE
- Journal :
- Journal of Molecular Biology
- Accession number :
- edsair.doi.dedup.....3573f92da0fa552dfebd3bb7194fc50c