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The effect of kisspeptin on spermatogenesis and apoptosis in rats
- Source :
- Volume: 47, Issue: 1 334-342, Turkish Journal of Medical Sciences
- Publication Year :
- 2017
- Publisher :
- TÜBİTAK, 2017.
-
Abstract
- Background/aim: To study the effect of kisspeptin, a gonadotropin release stimulator, on the testicular tissue of the rat. Materials and methods: Four groups were formed as follows: control, Kiss-10 50nmol administration for 1 day, Kiss-10 administration for 13 days, and one last group kept for 7 days following Kiss-10 applied for 13 days. Testicular tissues were stained with hematoxylineosin, periodic acid Schiff, Masson trichrome staining, terminal deoxynucleotidyl transferased UTP nick-end labeling, and Ki-67 immune staining. Serum testosterone levels were determined. Results: Serum testosterone level increased following acute application, while it was reduced by chronic treatment. Spermatogenic cells as stained by Ki-67 and TUNEL increased in the treated groups compared to the controls. Following a 7-day rest after treatment, a decrease in testosterone levels and Ki-67–stained cell numbers and an increase in TUNEL-stained cells were observed. Leydig cells showed increased vacuolization in the Kiss-1 group. Leydig cell vacuolization continued in the Kiss (13) group and was reduced in the Kiss (13 + 7) group. Conclusion: Kiss-10 increased spermatogenic cell proliferation, while testosterone level and proliferation decreased and apoptosis increased during the waiting period. Background/aim: To study the effect of kisspeptin, a gonadotropin release stimulator, on the testicular tissue of the rat. Materials and methods: Four groups were formed as follows: control, Kiss-10 50nmol administration for 1 day, Kiss-10 administration for 13 days, and one last group kept for 7 days following Kiss-10 applied for 13 days. Testicular tissues were stained with hematoxylineosin, periodic acid Schiff, Masson trichrome staining, terminal deoxynucleotidyl transferased UTP nick-end labeling, and Ki-67 immune staining. Serum testosterone levels were determined. Results: Serum testosterone level increased following acute application, while it was reduced by chronic treatment. Spermatogenic cells as stained by Ki-67 and TUNEL increased in the treated groups compared to the controls. Following a 7-day rest after treatment, a decrease in testosterone levels and Ki-67–stained cell numbers and an increase in TUNEL-stained cells were observed. Leydig cells showed increased vacuolization in the Kiss-1 group. Leydig cell vacuolization continued in the Kiss (13) group and was reduced in the Kiss (13 + 7) group. Conclusion: Kiss-10 increased spermatogenic cell proliferation, while testosterone level and proliferation decreased and apoptosis increased during the waiting period.
- Subjects :
- Male
0301 basic medicine
endocrine system
Kisspeptin
endocrine system diseases
medicine.drug_class
Apoptosis
030209 endocrinology & metabolism
urologic and male genital diseases
Andrology
Masson's trichrome stain
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Rats, Wistar
Spermatogenesis
Cells, Cultured
Testosterone
Cerrahi
Kisspeptins
Spermatogenic Cell
TUNEL assay
Leydig cell
urogenital system
business.industry
Leydig Cells
General Medicine
Kisspeptin,spermatogenesis,apoptosis,Leydig cells
Rats
030104 developmental biology
medicine.anatomical_structure
Vacuolization
Gonadotropin
business
human activities
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 13000144 and 13036165
- Database :
- OpenAIRE
- Journal :
- Volume: 47, Issue: 1 334-342, Turkish Journal of Medical Sciences
- Accession number :
- edsair.doi.dedup.....355c7124170f43ffb5b9bbaf71a96449