Back to Search
Start Over
Interactions between p27 and p88 replicase proteins of Red clover necrotic mosaic virus play an essential role in viral RNA replication and suppression of RNA silencing via the 480-kDa viral replicase complex assembly
- Source :
- Virology. (2):213-224
- Publisher :
- Elsevier Inc.
-
Abstract
- Red clover necrotic mosaic virus (RCNMV), a positive-sense RNA virus with a bipartite genome, encodes p27 and p88 replicase proteins that are required for viral RNA replication and suppression of RNA silencing. In this study, we indentified domains in p27 and p88 responsible for their protein–protein interactions using in vitro pull-down assays with the purified recombinant proteins. Coimmunoprecipitation analysis in combination with blue-native polyacrylamide gel electrophoresis using mutated p27 proteins showed that both p27–p27 and p27–p88 interactions are essential for the formation of the 480-kDa complex, which has RCNMV-specific RNA-dependent RNA polymerase activity. Furthermore, we found a good correlation between the accumulated levels of the 480-kDa complex and replication levels and the suppression of RNA silencing activity. Our results indicate that interactions between RCNMV replicase proteins play an essential role in viral RNA replication and in suppressing RNA silencing via the 480-kDa replicase complex assembly.
- Subjects :
- Gene Expression Regulation, Viral
RNA-induced transcriptional silencing
RNA-induced silencing complex
Coimmunoprecipitation analysis
Molecular Sequence Data
Trans-acting siRNA
RNA-dependent RNA polymerase
Dianthovirus
Virus Replication
Blue-native PAGE
Positive-sense RNA virus
Viral Proteins
chemistry.chemical_compound
In vitro translation and RNA synthesis
Transcription (biology)
RNA polymerase
Virology
Tombusviridae
Tobacco
Point Mutation
Amino Acid Sequence
Plant Diseases
Viral replicase complex
biology
Virus Assembly
Viral RNA replication
RNA virus
biology.organism_classification
Protein–protein interaction
RNA silencing
chemistry
RNA, Viral
RNA Interference
RNA silencing suppression
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....352a81417d173ba1779d084d3f9e32f4
- Full Text :
- https://doi.org/10.1016/j.virol.2010.07.038