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Common and unique Arabidopsis proteins involved in stomatal susceptibility to Salmonella enterica and Pseudomonas syringae
- Source :
- FEMS microbiology letters, vol 366, iss 16, FEMS Microbiol Lett
- Publication Year :
- 2019
- Publisher :
- Oxford University Press (OUP), 2019.
-
Abstract
- Salmonella enterica is one of the most common pathogens associated with produce outbreaks worldwide; nonetheless, the mechanisms uncovering their interaction with plants are elusive. Previous reports demonstrate that S. enterica ser. Typhimurium (STm), similar to the phytopathogen Pseudomonas syringae pv. tomato (Pst) DC3000, triggers a transient stomatal closure suggesting its ability to overcome this plant defense and colonize the leaf apoplast. In order to discover new molecular players that function in the stomatal reopening by STm and Pst DC3000, we performed an Arabidopsis mutant screening using thermal imaging. Further stomatal bioassay confirmed that the mutant plants exo70h4-3, sce1-3, bbe8, stp1, and lsu2 have smaller stomatal aperture widths than the wild type Col-0 in response to STm 14028s. The mutants bbe8, stp1 and lsu2 have impaired stomatal movement in response to Pst DC3000. These findings indicate that EXO70H4 and SCE1 are involved in bacterial-specific responses, while BBE8, STP1, and LSU2 may be required for stomatal response to a broad range of bacteria. The identification of new molecular components of the guard cell movement induced by bacteria will enable a better understanding of the initial stages of plant colonization and facilitate targeted prevention of leaf contamination with harmful pathogens.
- Subjects :
- 0106 biological sciences
Mutant
Arabidopsis
Pseudomonas syringae
Medical and Health Sciences
01 natural sciences
Microbiology
phytopathogen
03 medical and health sciences
Guard cell
Research Letter
Genetics
Plant defense against herbivory
Genetic Testing
Arabidopsis mutant screening
Molecular Biology
Plant Diseases
030304 developmental biology
0303 health sciences
Agricultural and Veterinary Sciences
biology
Arabidopsis Proteins
Prevention
Optical Imaging
fungi
Wild type
Salmonella enterica
food and beverages
foodborne illness
Biological Sciences
biology.organism_classification
Apoplast
Infectious Diseases
Emerging Infectious Diseases
Plant Stomata
Host-Pathogen Interactions
Biological Assay
stomatal movement
enterobacteria
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15746968 and 03781097
- Volume :
- 366
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Letters
- Accession number :
- edsair.doi.dedup.....352933dff9b45adb22f92146de674719