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Restriction fragment length polymorphisms associated with immunoglobulin C gamma genes reveal linkage disequilibrium and genomic organization
- Publication Year :
- 1983
-
Abstract
- We have demonstrated that restriction fragment length polymorphisms (RFLPs) produced by BamHI can be used as markers for constant (C) region heavy chain genes C psi gamma (C gamma pseudogene), C gamma 2, and C gamma 4. These RFLPs were found nonrandomly associated in the population sample studied. Of the eight combinations (haplotypes) of RFLPs theoretically possible, only two accounted for a total of 88% of the 116 chromosomes examined, a value greater than the total of 25% expected from random segregation of alleles. This indicates considerable linkage disequilibrium between C psi gamma, C gamma 2, and C gamma 4. Quantitative assessment of the degree of association between C gamma gene RFLPs, Gm markers, and switch region RFLPs adjacent to C mu and C alpha 1 revealed that C psi gamma is most tightly associated with C gamma 2 (r = 0.81 and 0.95 for the two common haplotypes), suggesting that C psi gamma maps to a position lying between C alpha 1 and C gamma 2. The association analysis used here should have general applicability for studying the genomic organization of other multigene families.
- Subjects :
- Genetics
Linkage disequilibrium
Multidisciplinary
Polymorphism, Genetic
Genetic Linkage
Immunoglobulin gamma-Chains
Haplotype
Immunoglobulins
Nucleic Acid Hybridization
DNA Restriction Enzymes
Biology
Molecular biology
Genes
Genetic linkage
Humans
BamHI
Restriction fragment length polymorphism
Immunoglobulin Constant Regions
Immunoglobulin Heavy Chains
Alleles
Genetic association
Genomic organization
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....3528dd761dd6b25bfbe968de6867511a