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Combination of Cytokine Responses Indicative of Latent TB and Active TB in Malawian Adults
- Source :
- PLoS ONE, 8(11), PLoS ONE, PLoS ONE, Vol 8, Iss 11, p e79742 (2013), PLoS ONE; Vol 8
- Publication Year :
- 2013
-
Abstract
- BACKGROUND An IFN-γ response to M. tuberculosis-specific antigens is an effective biomarker for M. tuberculosis infection but it cannot discriminate between latent TB infection and active TB disease. Combining a number of cytokine/chemokine responses to M. tuberculosis antigens may enable differentiation of latent TB from active disease. METHODS Asymptomatic recently-exposed individuals (spouses of TB patients) were recruited and tuberculin skin tested, bled and followed-up for two years. Culture supernatants, from a six-day culture of diluted whole blood samples stimulated with M. tuberculosis-derived PPD or ESAT-6, were measured for IFN-γ, IL-10, IL-13, IL-17, TNF-α and CXCL10 using cytokine ELISAs. In addition, 15 patients with sputum smear-positive pulmonary TB were recruited and tested. RESULTS Spouses with positive IFN-γ responses to M. tuberculosis ESAT-6 (>62.5 pg/mL) and TB patients showed high production of IL-17, CXCL10 and TNF-α. Higher production of IL-10 and IL-17 in response to ESAT-6 was observed in the spouses compared with TB patients while the ratios of IFN-γ/IL-10 and IFN-γ/IL-17 in response to M. tuberculosis-derived PPD were significantly higher in TB patients compared with the spouses. Tuberculin skin test results did not correlate with cytokine responses. CONCLUSIONS CXCL10 and TNF-α may be used as adjunct markers alongside an IFN-γ release assay to diagnose M. tuberculosis infection, and IL-17 and IL-10 production may differentiate individuals with LTBI from active TB.
- Subjects :
- Adult
Male
Malawi
Tuberculosis
Tuberculin
lcsh:Medicine
Asymptomatic
Mycobacterium tuberculosis
03 medical and health sciences
Interferon-gamma
0302 clinical medicine
Antigen
Bacterial Proteins
Latent Tuberculosis
medicine
CXCL10
Humans
Prospective Studies
lcsh:Science
030304 developmental biology
0303 health sciences
Antigens, Bacterial
Multidisciplinary
biology
business.industry
Tuberculin Test
Tumor Necrosis Factor-alpha
Interleukins
lcsh:R
Sputum
biology.organism_classification
medicine.disease
3. Good health
Chemokine CXCL10
Immunology
Biomarker (medicine)
lcsh:Q
Female
medicine.symptom
business
030215 immunology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, 8(11), PLoS ONE, PLoS ONE, Vol 8, Iss 11, p e79742 (2013), PLoS ONE; Vol 8
- Accession number :
- edsair.doi.dedup.....352167aff2f4832ae7af2b0bf321b78b