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Identification and characterization of a novel C20-elongase gene from the marine microalgae, Pavlova viridis, and its use for the reconstitution of two pathways of long-chain polyunsatured fatty acids biosynthesis in Saccharomyces cerevisiae
- Source :
- Biotechnology letters. 35(8)
- Publication Year :
- 2013
-
Abstract
- The marine microalga, Pavlova viridis, contains long-chain polyunsatured fatty acids including eicosapentaenoic acid (EPA, 20:5n-3) and docosapentaenoic acid (DPA, 22:5n-3). A full-length cDNA sequence, pvelo5, was isolated from P. viridis. From sequence alignment, the gene was homologous to fatty acyl elongases from other organisms. Heterologous expression of pvelo5 in Saccharomyces cerevisiae confirmed that it encoded a specific C20-elongase within the n-3 and n-6 pathways. Elongation activity was confined exclusively to EPA and arachidonic acid (20:4n-6). GC analysis indicated that pvelo5 could co-express with other genes for biosynthesis to reconstitute the Δ8 and Δ6 pathways. Real-time PCR results and fatty acid analysis demonstrated that long-chain polyunsatured fatty acids production by the Δ8 pathway might be more effective than that by the Δ6 pathway.
- Subjects :
- Sequence analysis
Fatty Acid Elongases
Saccharomyces cerevisiae
Molecular Sequence Data
Gene Expression
Bioengineering
Applied Microbiology and Biotechnology
Gas Chromatography-Mass Spectrometry
chemistry.chemical_compound
DNA, Algal
Biosynthesis
Acetyltransferases
chemistry.chemical_classification
biology
Fatty Acids
Fatty acid
Haptophyta
General Medicine
Sequence Analysis, DNA
biology.organism_classification
Eicosapentaenoic acid
Recombinant Proteins
Biosynthetic Pathways
Biochemistry
chemistry
Metabolic Engineering
Arachidonic acid
Docosapentaenoic acid
Heterologous expression
Biotechnology
Subjects
Details
- ISSN :
- 15736776
- Volume :
- 35
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Biotechnology letters
- Accession number :
- edsair.doi.dedup.....3511bca6c6110fb8ede083cab0819f8c