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Increased uptake of oxidized LDL by macrophages from type 2 diabetics is inhibited by polyamines
- Source :
- Biomedicine & Pharmacotherapy. 77:59-64
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The aim of this study was to evaluate the effect of polyamines putrescine, spermidine and spermine on human LDL oxidation and to assess the ability of macrophages derived from type 2 diabetic patients to uptake oxLDL. Polyamine effect was compared with α-tocopherol. Four healthy subjects and eight type 2 diabetic patients were included in this study. To characterize type 2 diabetic patients and non-diabetic subjects, laboratory test were carried out. Glucose, glycated haemoglobin (HbA1C), triglycerides, low (LDL) and high density lipoproteins (HDL) and serum lipid peroxidation were measured in blood. The study was performed in three stages. For each stage, ten experimental conditions comparing the effect of polyamines with α-tocopherol (10μM solutions) on LDL oxidation and the uptake of oxLDL by macrophages were analyzed. MDA concentration was found to be significantly higher in type 2 diabetic patients compared to healthy subjects (5.6±0.58 vs. 2.66±0.31μM MDA, respectively, (P
- Subjects :
- Blood Glucose
Male
0301 basic medicine
medicine.medical_specialty
Antioxidant
Spermidine
medicine.medical_treatment
alpha-Tocopherol
Spermine
Antioxidants
Lipid peroxidation
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Lipid oxidation
Internal medicine
Diabetes mellitus
Polyamines
Putrescine
medicine
Humans
Glycated Hemoglobin
Pharmacology
business.industry
Macrophages
General Medicine
Middle Aged
medicine.disease
Lipids
Lipoproteins, LDL
030104 developmental biology
Endocrinology
Diabetes Mellitus, Type 2
chemistry
Biochemistry
030220 oncology & carcinogenesis
Female
lipids (amino acids, peptides, and proteins)
Lipid Peroxidation
Polyamine
business
Subjects
Details
- ISSN :
- 07533322
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Biomedicine & Pharmacotherapy
- Accession number :
- edsair.doi.dedup.....350a31e1412dd8bc971b72913d0dc003