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Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation
- Source :
- Cell & Bioscience, Cell & Bioscience, Vol 12, Iss 1, Pp 1-17 (2022)
- Publication Year :
- 2022
- Publisher :
- BioMed Central, 2022.
-
Abstract
- Background During male meiosis, the Y chromosome can form perfect pairing with the X chromosome. However, it is unclear whether mammalian Female germline stem cells (FGSCs) without a Y chromosome can transdifferentiate into functional haploid spermatid-like cells (SLCs). Results We found that spermatogenesis was restarted by transplanting FGSCs into Kitw/wv mutant testes. Complete meiosis and formation of SLCs was induced in vitro by testicular cells of Kitw/wv mutant mice, cytokines and retinoic acid. Healthy offspring were produced by sperm and SLCs derived from the in vivo and in vitro transdifferentiation of FGSCs, respectively. Furthermore, high-throughput chromosome conformation capture sequencing(Hi-C-seq) and “bivalent” (H3K4me3-H3K27me3) micro chromatin immunoprecipitation sequencing (μChIP-seq) experiments showed that stimulated by retinoic acid gene 8 (STRA8)/protamine 1 (PRM1)-positive transdifferentiated germ cells (tGCs) and male germ cells (mGCs) display similar chromatin dynamics and chromatin condensation during in vitro spermatogenesis. Conclusion This study demonstrates that sperm can be produced from FGSCs without a Y chromosome. This suggests a strategy for dairy cattle breeding to produce only female offspring with a high-quality genetic background.
- Subjects :
- Transdifferentiation
QH301-705.5
Research
Bivalent
Female germline stem cells
Spermatid-like cells
QD415-436
Biochemistry
General Biochemistry, Genetics and Molecular Biology
Biology (General)
High-throughput chromosome conformation capture sequencing
TP248.13-248.65
In vitro spermatogenesis
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 20453701
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Cell & Bioscience
- Accession number :
- edsair.doi.dedup.....34f5323956a54f16ad46ef8b3e8e25d4