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Inflammatory Bowel Disease Therapies and Gut Function in a Colitis Mouse Model
- Source :
- BioMed Research International, Vol 2013 (2013), BioMed Research International
- Publication Year :
- 2013
- Publisher :
- Hindawi Limited, 2013.
-
Abstract
- Background. Exclusive enteral nutrition (EEN) is a well-established approach to the management of Crohn’s disease.Aim. To determine effects of EEN upon inflammation and gut barrier function in a colitis mouse model.Methods. Interleukin-10-deficient mice (IL-10−/−) were inoculated withHelicobacter trogontumand then treated with EEN, metronidazole, hydrocortisone, or EEN and metronidazole combination. Blood and tissue were collected at 2 and 4 weeks with histology, mucosal integrity, tight junction integrity, inflammation, andH. trogontumload evaluated.Results.H. trogontuminduced colitis in IL-10−/−mice with histological changes in the cecum and colon. Elevated mucosal IL-8 mRNA in infected mice was associated with intestinal barrier dysfunction indicated by decreased transepithelial electrical resistance and mRNA of tight junction proteins and increased short-circuit current, myosin light chain kinase mRNA, paracellular permeability, and tumor necrosis factor-αand myeloperoxidase plasma levels (P<0.01for all comparisons). EEN and metronidazole, but not hydrocortisone, treatments restored barrier function, maintained gut barrier integrity, and reversed inflammatory changes along with reduction ofH. trogontumload (versus infected controlsP<0.05).Conclusion.H. trogontuminfection in IL-10−/−mice induced typhlocolitis with intestinal barrier dysfunction. EEN and metronidazole, but not hydrocortisone, modulate barrier dysfunction and reversal of inflammatory changes.
- Subjects :
- medicine.medical_specialty
Article Subject
lcsh:Medicine
Inflammation
Inflammatory bowel disease
Gastroenterology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Cecum
Mice
0302 clinical medicine
Enteral Nutrition
Intestinal mucosa
Crohn Disease
Internal medicine
Helicobacter
Metronidazole
medicine
Animals
Humans
RNA, Messenger
Colitis
Intestinal Mucosa
Barrier function
030304 developmental biology
0303 health sciences
General Immunology and Microbiology
biology
lcsh:R
General Medicine
Helicobacter trogontum
biology.organism_classification
medicine.disease
Inflammatory Bowel Diseases
3. Good health
Interleukin-10
Disease Models, Animal
medicine.anatomical_structure
030211 gastroenterology & hepatology
medicine.symptom
medicine.drug
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2013
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....34eac783e2e57525a66d30a91ef0df3a