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Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL
- Publication Year :
- 2016
-
Abstract
- The interaction between the TNF-family molecule receptor activator of NF-kappaB ligand (RANKL) and its receptor RANK induces osteoclast formation, activation and survival in the process of bone remodelling. RANKL-RANK also plays critical roles in T-cell/dendritic cell communication and lymph-node formation and in a variety of pathologic conditions such as tumour-cell migration and bone metastasis. Both the ectodomain of mouse RANKL and the extracellular domain of mouse RANK have been cloned, expressed and purified. Crystals of RANK alone and of RANK in complex with RANKL have been obtained that are suitable for structure determination.
- Subjects :
- musculoskeletal diseases
Protein Folding
Rotation
Recombinant Fusion Proteins
Genetic Vectors
Molecular Sequence Data
Statistics as Topic
Cell
Biophysics
Biochemistry
Mice
X-Ray Diffraction
Structural Biology
Osteoclast
Escherichia coli
Genetics
medicine
Animals
Histidine
Amino Acid Sequence
Cloning, Molecular
Receptor
Glutathione Transferase
Inclusion Bodies
Receptor Activator of Nuclear Factor-kappa B
biology
Activator (genetics)
Chemistry
Data Collection
RANK Ligand
Dendritic cell
Condensed Matter Physics
Protein Structure, Tertiary
Cell biology
Molecular Weight
medicine.anatomical_structure
Solubility
Ectodomain
Crystallization Communications
RANKL
biology.protein
Crystallization
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....34d8755ac1c896286522e36ac8815c8d