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2-Arachidonoylglycerol increases in ischemia-reperfusion injury of the rat liver

Authors :
Yasuo Morishita
Ikuro Maruyama
Izumi Takeyoshi
Daisuke Yoshinari
Koshi Matsumoto
Makoto Kurabayashi
Source :
Journal of investigative surgery : the official journal of the Academy of Surgical Research. 18(1)
Publication Year :
2005

Abstract

Several studies have implicated endocannabinoids in various forms of shock. However, the role of endocannabinoids in hepatic ischemia-reperfusion injury remains unclear. The purpose of this study was to evaluate the changes of two endocannabinoidsin hepatic ischemia-reperfusion injury: anandamide (ANA) and 2-arachidonoylglycerol (2-AG). Male Sprague-Dawley rats were divided into 2 groups: the short (15 min) ischemic group and the long (60 min)ischemic group in the segmental (70%) hepatic tissue. Blood levels of aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), ANA, and 2-AG were examined. Serum lev-els of AST, ALT, and LDH were significantly higher in the long-ischemia group than in the short-ischemia group. Plasma levels of 2-AG showed similar augmentation prior to and after reperfusion in both the short- and long-ischemia groups, although plasma 2-AG lev-els tended to be higher in the long-ischemia group than in the short-ischemia group. Plasma levels of ANA were augmented in the early phase of reperfusion in the short-ischemia group and did not differ significantly from the normal level with time after reperfusion in the long-ischemia group. These results suggest that the endocannabinoid 2-AG increases in hepatic ischemia-reperfusion injury of rats, rather than ANA.

Details

ISSN :
08941939
Volume :
18
Issue :
1
Database :
OpenAIRE
Journal :
Journal of investigative surgery : the official journal of the Academy of Surgical Research
Accession number :
edsair.doi.dedup.....34bbeafc708343014b0675285bf87f5c