Back to Search
Start Over
Characterization of a new transferable MDR plasmid carrying thepbp5gene from a clade B commensalEnterococcus faecium
- Source :
- Journal of Antimicrobial Chemotherapy. 74:843-850
- Publication Year :
- 2019
- Publisher :
- Oxford University Press (OUP), 2019.
-
Abstract
- OBJECTIVES To evaluate the transferability of antibiotic resistance from an MDR clade B Enterococcus faecium and to characterize the genetic elements involved. METHODS The erm(B)-positive strain E. faecium 37BA (donor) and strains E. faecium 64/3 and Listeria welshimeri 11857RF (recipients) were used in mating experiments. Donors and transconjugants were characterized using MIC assays, PFGE, Southern blotting and hybridization, quantitative RT-PCR (RT-qPCR), next-generation sequencing and PCR mapping. RESULTS One E. faecium and one L. welshimeri transconjugant were selected for in-depth investigation. Both acquired an ∼40 kb plasmid carrying erm(B). An additional plasmid of ∼200 kb, encoding the full conjugation machinery, was detected in the donor and in the E. faecium transconjugant. Next-generation sequencing revealed a new 40 396 bp plasmid that was designated pEf37BA; it contained 10 antibiotic resistance genes, tet(M), tet(L), erm(B), aadE, sat4, aphA, spw, lsa(E), lnu(B) and pbp5, resulting from the recombination of pM7M2 of E. faecium with an MDR chromosomal region of Erysipelothrix rhusiopathiae. A pbp5-carrying circular form was also detected. The PBP5 amino acid sequence differed from the C46 variant by two mutations (S39T and D644N). Its expression was documented in both transconjugants. pEf37BA persisted in the absence of selective pressure. CONCLUSIONS The MDR clade B E. faecium plasmid, deriving from the recombination of two different resistance regions, carried a pbp5 element and was transferable to different bacterial species. This finding further documents the dissemination of ampicillin resistance among community-associated E. faecium and the key role of commensal strains in the spread of antibiotic resistance.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Gene Transfer, Horizontal
Genotype
Listeria
Enterococcus faecium
030106 microbiology
Microbial Sensitivity Tests
Real-Time Polymerase Chain Reaction
Microbiology
law.invention
03 medical and health sciences
0302 clinical medicine
Plasmid
Antibiotic resistance
Amp resistance
law
Drug Resistance, Multiple, Bacterial
Humans
Penicillin-Binding Proteins
Pharmacology (medical)
030212 general & internal medicine
Polymerase chain reaction
Southern blot
Pharmacology
biology
Chromosome Mapping
High-Throughput Nucleotide Sequencing
Methyltransferases
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Healthy Volunteers
Anti-Bacterial Agents
Electrophoresis, Gel, Pulsed-Field
Blotting, Southern
Infectious Diseases
Listeria welshimeri
Genes, Bacterial
Conjugation, Genetic
Chromosomal region
Plasmids
Subjects
Details
- ISSN :
- 14602091 and 03057453
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy
- Accession number :
- edsair.doi.dedup.....34abc8a837901e0dd1d41c37c13e8ec4