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Predicting the ligand-binding properties of Borrelia burgdorferi s.s. Bmp proteins in light of the conserved features of related Borrelia proteins
- Source :
- Journal of Theoretical Biology. 462:97-108
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Bacteria of the genus Borrelia cause vector-borne infections like the most important hard tick-borne disease in the northern hemisphere, Lyme borreliosis (LB), and soft tick or louse transmitted relapsing fevers (RF), prevalent in temperate and tropical areas. Borrelia burgdorferi sensu lato (s.l.) includes several genospecies and causes LB in humans. In infected patients, Borrelia burgdorferi sensu stricto (s.s.) expresses the BmpA, BmpB, BmpC and BmpD proteins. The role of these proteins in the pathogenesis of LB remains incompletely characterized, but they are, however, closely related to Treponema pallidum PnrA (Purine nucleoside receptor A), a substrate-binding lipoprotein of the ATP-binding cassette (ABC) transporter family preferentially binding purine nucleosides. Based on 3D homology modeling, the Bmp proteins share the typical fold of the substrate-binding protein family and the ligand-binding properties of BmpA, BmpB and BmpD are highly similar, whereas those of BmpC differ markedly. Nevertheless, these residues are highly conserved within the genus Borrelia and the inferred phylogenetic tree also reveals that the RF Borrelia lack BmpB proteins but has an additional Bmp protein (BmpA2) missing in LB-causing Borrelia burgdorferi s.l. Our results indicate that the Bmp proteins could bind nucleosides, although BmpC might have a different ligand-binding specificity and, therefore, a distinct function. Furthermore, the work provides a means for classifying the Bmp proteins and supports further elucidation of the roles of these proteins.
- Subjects :
- 0301 basic medicine
Statistics and Probability
Protein family
ta3111
Ligands
General Biochemistry, Genetics and Molecular Biology
Microbiology
03 medical and health sciences
0302 clinical medicine
Bacterial Proteins
Phylogenetics
Borrelia
Humans
Homology modeling
Borrelia burgdorferi
Receptor
Lyme Disease
Treponema
General Immunology and Microbiology
biology
Phylogenetic tree
Applied Mathematics
Nucleosides
General Medicine
bacterial infections and mycoses
biology.organism_classification
030104 developmental biology
Structural Homology, Protein
Modeling and Simulation
General Agricultural and Biological Sciences
030217 neurology & neurosurgery
Protein Binding
Subjects
Details
- ISSN :
- 00225193
- Volume :
- 462
- Database :
- OpenAIRE
- Journal :
- Journal of Theoretical Biology
- Accession number :
- edsair.doi.dedup.....347e5da9eb699713baa84b6e4f2ea1f2
- Full Text :
- https://doi.org/10.1016/j.jtbi.2018.11.004